| 应用大转瓶结合微载体培养大量生产疫苗的研究 |
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| 引用本文： | 陶全富,胡奎,金扩世,董爱鱼,殷震. 应用大转瓶结合微载体培养大量生产疫苗的研究[J]. 中国兽医学报, 1986, 0(4) |
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| 作者姓名： | 陶全富 胡奎 金扩世 董爱鱼 殷震 |
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| 作者单位： | 中国人民解放军兽医大学军事兽医研究所二室(陶全富，胡奎，金扩世，董爱鱼)，中国人民解放军兽医大学军事兽医研究所二室(殷震) |
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| 摘 要： | 在普通转瓶细胞培养系统中加入微载体进行细胞培养试验。在几种微载体和营养液培养比较试验的基础上,对转瓶单层培养、转瓶载体培养及转瓶单层培养加载体培养三种方法进行了比较。进而应用10000ml大转瓶,用牛胎皮肤继代细胞、IBRS-2细胞,以效果较好的转瓶单层培养加载体培养的方法,进行了病毒疫苗生产试验。经3个毒株、8个大转瓶培养试验,证明培养量可增加1倍,而且TCID_(50)/ml均在8.0以上,方法简便易行,无需特殊的设备与条件。
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| 关 键 词： | 微载体 转瓶单层培养 转瓶载体培养 |
A STUDY ON COMBINED USE OF ROLLER BOTTLE AND MICROCARRIER CELL CULTURE SYSTEM IN VACCINE PRODUCTION |
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| Tao Quanfu et al. Second laboratory of Military Veterinary Institute,Veterinary College of PLA. A STUDY ON COMBINED USE OF ROLLER BOTTLE AND MICROCARRIER CELL CULTURE SYSTEM IN VACCINE PRODUCTION[J]. Chinese Journal of Veterinary Science, 1986, 0(4) |
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| Authors: | Tao Quanfu et al. Second laboratory of Military Veterinary Institute Veterinary College of PLA |
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| Abstract: | Various methods of cell culture and their requirments werecompared. A new technique using big roller bottle and microcarrier systemwas developed. The technique is time and labour saving and not has toresort to special equipment and ingenious procedure. The yield of cultured cell and thus the titer of virus were higher than those of simple roller cellculture. Bovine skin cells and IBRS-2 cells were successfully grown onmicrocarrier culture in roller bottle, the yield of virus was 100 % greaterthan the yield of simple roller bottle and the infective titer of the virusharvest reached to 8.0 log_(10) ID_(50)/ml. Microcarrier culture in roller bottleseems to be as a potential way for the mass production of virus vaccine. |
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| Keywords: | microcarrier monolayer culture using roller bottle bolle roller and microcarrier culture |
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