| 栗疫病菌单个营养体不亲和性基因差异对弱毒性病毒传递的影响 |
| |
| 引用本文： | 李涛,华朝晖,林少波,兰春能,许利强,乔广行,邓清超,燕娜娜,王克荣. 栗疫病菌单个营养体不亲和性基因差异对弱毒性病毒传递的影响[J]. 南京农业大学学报, 2009, 32(2) |
| |
| 作者姓名： | 李涛 华朝晖 林少波 兰春能 许利强 乔广行 邓清超 燕娜娜 王克荣 |
| |
| 作者单位： | 1. 南京农业大学植物保护学院,江苏,南京,210095
2. 浙江省遂昌县林业技术推广总站,浙江,丽水,323300 |
| |
| 基金项目： | 国家自然科学基金，浙江省重大科技专项(优先主题)农业项目 |
| |
| 摘 要： | 研究了栗疫病菌(Cryphonectria parasitica)菌株间单个营养体不亲和性(vegetative incompatibility,vic)基因差异对弱毒性病毒传递的影响.将含病毒菌株与具单个vic基因座位差异的野生型菌株配对培养,10 d后调查菌株间的病毒传递率.结果表明,相同vic基因型菌株间的病毒传递率为100%;在1个vic座位上的基因不同通常会降低病毒的传递率,在vic-Cc座位上基因差异菌株间的病毒平均传递率为85.93%,而在vic-Dd座位上基因不同菌株间病毒的平均传递率仅为10.30%,在vic-Aa、vic-Bb座位上基因不同的菌株间病毒平均传递率分别为44.12%、47.93%.vic-Bb座位上基因不同菌株间的病毒正反向传递率相差非常大,由vic-b菌株向vic-B菌株的平均病毒传递率为83.13%,而从vic-B菌株向vic-b菌株的平均病毒传递率只有23.15%;其他3个vic座位上基因不同菌株间的病毒传递率也具有方向性,但不明显.
|
| 关 键 词： | 栗疫病菌 营养体不亲和性 vic基因 传递 弱毒性病毒 |
Effects of one different vic genes on transmission of hypovirus in Cryphonectria parasitica |
| |
| LI Tao,HUA Chao-hui,LIN Shao-bo,LAN Chun-neng,XU Li-qiang,QIAO Guang-hang,DENG Qing-chao,YAN Na-na,WANG Ke-rong. Effects of one different vic genes on transmission of hypovirus in Cryphonectria parasitica[J]. Journal of Nanjing Agricultural University, 2009, 32(2) |
| |
| Authors: | LI Tao HUA Chao-hui LIN Shao-bo LAN Chun-neng XU Li-qiang QIAO Guang-hang DENG Qing-chao YAN Na-na WANG Ke-rong |
| |
| Affiliation: | 1.College of Plant Protection;Nanjing Agricultural University;Nanjing 210095;China;2.Zhejiang Province Suichang General Station of Forest Technology;Lishui 323300;China |
| |
| Abstract: | Effect of heteroallelism at each vegetative incompatibility(vic)locus on transmission of hypovirus in Cryphonectria parasitica was characterized.The 16 isolates of different genotype at 4 vic loci were obtained through sexsual crossing between isolates which have known genotypes.Virus transmission was assayed by growing donor and recipient isolates together.Virus transmission occurs with 100% frequency when donor and recipient isolates have the same vic genotypes but heteroallelism at one of these loci gene... |
| |
| Keywords: | |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
