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| 江西地方猪种SLA-DRB基因外显子2的多态性研究 | |
| 引用本文： | 鞠慧萍,吴圣龙,包文斌,程金花,李碧春,陈国宏,杨建生,黄雪根,华金弟.江西地方猪种SLA-DRB基因外显子2的多态性研究[J].畜牧与兽医,2007,39(7):4-6. |
| 作者姓名： | 鞠慧萍 吴圣龙 包文斌 程金花 李碧春 陈国宏 杨建生 黄雪根 华金弟 |
| 作者单位： | 1. 扬州大学动物科学与技术学院,江苏,扬州,225009 2. 扬州大学动物科学与技术学院,江苏,扬州,225009;安徽农业大学动物科技学院,安徽,合肥,2300361 3. 江苏省昆山市畜牧兽医站,江苏,昆山,215300 4. 江苏省苏州市苏太猪育种中心,江苏,苏州,215128 |
| 基金项目： | 江苏省高技术研究发展计划项目(BG2006302)，扬州大学自然科学基金资助项目(NK0513119) |
| 摘 要： | 采用PCR-RFLP法对3个江西地方猪种(乐平花猪、修水杭猪和万安花猪)的SLA-DRB基因外显子2的PCR产物进行多态性分析。通过限制型内切酶RsaⅠ酶切后,共产生9种组合带型。通过检测发现:乐平花猪有8种组合带型,即AA、AB、AC、BB、BC、BD、CD和DD;修水杭猪有3种组合带型,即AA、AB和AC;万安花猪有4种组合带型,即AA、AC、BC和CC,且3个猪种中均以A为优势带型,在猪种中频率相差不大。χ2检测表明,乐平花猪和修水杭猪SLA-DRB基因外显子2在RsaⅠ酶切位点达到了Hardy-Weinberg平衡状态,而万安花猪未达到Hardy-Weinberg平衡状态。 |
| 关 键 词： | 地方猪种 SLA-DRB PCR-RFLP |
| 文章编号： | 0529-5130（2007）07-0004-03 |
| 修稿时间： | 2006-08-26 |
Polymorphism analysis of the exon 2 of SLA-DRB gene in three native pig breeds of Jiangxi province |
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| JU Hui-ping,WU Sheng-long,BAO Wen-bin,CHENG Jin-hua,LI Bi-chun,CHEN Guo-hong,YANG Jian-sheng,HUANG Xue-gen,HUA Jin-di.Polymorphism analysis of the exon 2 of SLA-DRB gene in three native pig breeds of Jiangxi province[J].Animal Husbandry & Veterinary Medicine,2007,39(7):4-6. | |
| Authors: | JU Hui-ping WU Sheng-long BAO Wen-bin CHENG Jin-hua LI Bi-chun CHEN Guo-hong YANG Jian-sheng HUANG Xue-gen HUA Jin-di |
| Abstract: | The exon 2 of SLA-DRB gene in three native pig breeds of Jiangxi province was analysed by PCR-RFLP method.The results showed that the exon 2 digested by RsaⅠcould be divided into 9 kinds of combined patterns.All combined patterns CC were observed in Leping Spotted pig;AA,AB and AC in Hang pig,AA,AC,BC and CC in Wan'an Spotted pig.In each breed,A was the predominant pattern,and there were no significant differences in the frequency of A among three breeds.The results of Chi Square test indicated that the frequencies of patterns digested by RsaⅠwere Hardy-Weinberg equilibrium in Leping Spotted and hang pig populations(P>0.05). |
| Keywords: | Native pigs SLA-DRB PCR-RFLP |
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