Cell wall degrading enzymes for silage. 2. Aerobic stability of enzyme-treated laboratory silages |
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Authors: | I SELMER-OLSEN A R HENDERSON† S ROBERTSON† R McGINN† |
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Institution: | Agricultural University of Norway, Aas, Norway;The Scottish Agricultural College, Edinburgh, UK |
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Abstract: | The effects of two commercial cellulase/hemicellulase enzymes derived from Trichoderma reesei on silage fermentation and aerobic stability were investigated in three laboratory-scale experiments. In Experiment 1, perennial ryegrass ( Lolium perenne ) was treated with enzyme A at the rates of 0. 0·125, 0·250, 0·500 and 0·750 cm3 kg-1. In Experiment 2, Italian ryegrass ( Lolium multiflorum ) was treated with the same enzyme at the rates of 0, 0·250 and 0·500 cm3 kg-1 and with 85% formic acid (3·5 cm3 kg-1). In Experiment 3, perennial ryegrass was ensiled untreated, with enzyme A (0·250 and 0·500 cm3 kg-1) and with 0·200 and 0·400 cm3 kg-1 enzyme B which also contained glucose oxidase. All silages were well preserved. Aerobic deterioration was related to numbers of lactate assimilating yeasts. In Experiment 1, all silages were stable over a 4-day period. In Experiment 2, enzyme A treatment delayed the temperature rise compared with the untreated control ( P <0·05), whereas the formic acid-treated silage was stable. In Experiment 3, the temperature rise in the silage treated with the higher level of enzyme B occurred one day later than in the other silages ( P <0·05). |
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