Application of an orcinol-ferric chloride colorimetric assay in barley and wheat accessions for water-extractable and total arabinoxylan |
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| Institution: | 1. University of Manitoba, Department of Food Science, Winnipeg, Manitoba, Canada R3T 2N2;2. Canadian Grain Commission, Winnipeg, Manitoba, Canada R3C 3G8;3. University of Manitoba, Richardson Centre for Functional Foods & Nutraceuticals, Winnipeg, Manitoba, Canada R3T 2N2;1. Departamento de Nutrición Animal, Instituto de Producción Animal de la Facultad de Veterinaria, Universidad de la República, Ruta 1, km 42,500, Libertad, San José, Uruguay;2. Departamento de Bovinos, Instituto de Producción Animal de la Facultad de Veterinaria, Universidad de la República, Ruta 1, km 42,500, Libertad, San José, Uruguay;1. USDA-ARS, 1691 S 2700 W, Aberdeen, ID 83210, USA;2. Department of Plant, Soil, and Entomological Sciences, Program of Microbiology, Molecular Biology and Biochemistry, University of Idaho, Moscow, ID 83844, USA;1. Laboratory of Adaptive and Regenerative Biology, Brigham & Women’s Hospital, Harvard Medical School, Boston, MA, United States;2. Department of Diagnostic and Interventional Radiology, Translational Lung Research Center, University of Heidelberg, Heidelberg, Germany;3. Joint BioEnergy Institute, Emeryville CA and the Environmental Genomics and Systems Biology Division, Lawrence Berkeley National Laboratory, Berkeley, CA, United States;4. Complex Carbohydrate Research Center and Department of Biochemistry and Molecular Biology, University of Georgia, Athens, GA, United States;5. Institute of Functional and Clinical Anatomy, University Medical Center of the Johannes Gutenberg-University, Mainz, Germany |
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| Abstract: | Arabinoxylan is an important hemicellulose potentially affecting wheat baking qualities, barley malt quality, and may impart prebiotic benefits. Water-extractable (WEAX) and total arabinoxylan (TOAX) were characterized in 204 wheat and barley accessions, respectively, using an orcinol-ferric chloride assay. WEAX measurement accuracy was optimum when glucose concentration was greater than 12–13 times pentose concentration. The modified method removed enough excess glucose spectral absorbance to make the corrected and uncorrected lines different, but still significantly correlated (p = 0.009, R = 0.94). Mean WEAX (expressed as percentage WEAX of TOAX) for the wheat accessions was 15.90%, ranging from 8.82% to 24.87%, and for barley accessions WEAX the mean was 7.10%, and ranged from 2.98% to 13.86%. Conclusions are 1) the assay is useful for a breeding program because of its semi-high throughput design for the simultaneous analysis of 16 (WEAX) to 40 (TOAX) barley lines or 24 (WEAX) to 40 (TOAX) wheat lines, 2) the trichromatic measurement reduces the impact of glucose, and 3) the broad range of WEAX measured showed that barley and wheat accessions vary in their extractable and unextractable components. |
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| Keywords: | Arabinoxylan Orcinol Colorimetry Wheat Barley Pentose WEAX WUAX TOAX Ferric chloride Trichromatic AX"} {"#name":"keyword" "$":{"id":"kwrd0070"} "$$":[{"#name":"text" "_":"arabinoxylan WEAX"} {"#name":"keyword" "$":{"id":"kwrd0080"} "$$":[{"#name":"text" "_":"water extractable arabinoxylan WUEX"} {"#name":"keyword" "$":{"id":"kwrd0090"} "$$":[{"#name":"text" "_":"water unextractable arabinoxylan TOAX"} {"#name":"keyword" "$":{"id":"kwrd0100"} "$$":[{"#name":"text" "_":"total arabinoxylan |
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