GWAS for resistance against black point caused by Bipolaris sorokiniana in wheat |
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| Affiliation: | 1. Division of Life Sciences, Korea University, Seoul 136-701, Republic of Korea;2. Environment and Metabolomics Research Team, Korea Basic Science Institute, Seoul 136-701, Republic of Korea;1. The University of Adelaide, Waite Campus, School of Agriculture Food and Wine, Urrbrae, South Australia, 5064, Australia;2. South Australian Research and Development Institute, Waite Precinct, Urrbrae, South Australia, 5064, Australia;1. School of Agronomy, Anhui Agricultural University, Hefei 230036, China;2. Key Laboratory of Wheat Biology and Genetic Improvement on South Yellow and Huai River Valley, Ministry of Agriculture, Hefei 230036, China;3. National United Engineering Laboratory for Crop Stress Resistance Breeding, Hefei 230036, China;4. Anhui Key Laboratory of Crop Biology, Hefei 230036, China;1. College of Horticulture, Henan Agricultural University, Zhengzhou 450002, China;2. Henan Key Laboratory of Fruit and Cucurbit Biology, Zhengzhou 450002, China;3. Xinyang Agriculture and Forestry University, Xinyang 464006, China;1. Department of Agricultural-Biotechnology, Sabzevar Branch, Islamic Azad University, Sabzevar, Iran;2. Plant Protection Research Department, Isfahan Agriculture and Natural Resource Research and Education Center, AREEO, Isfahan, 81786-96446, Iran;3. Department of Biology, Faculty of Science, Hakim Sabzevari University, Sabzevar, Iran;4. Horticulture Crop Research Department, Isfahan Agriculture and Natural Resource Research and Education Center, AREEO, Isfahan, 81786-96446, Iran |
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| Abstract: | Black point is a severe wheat grain disease caused by complex pathogens, of which Bipolaris sorokiniana is dominant. Analysis of effective resistance genes/quantitative trait loci (QTL) is an essential prerequisite for breeding by marker-assisted selection (MAS). In this study, we aimed to identify the loci resistant to black point caused by B. sorokiniana in 10 wheat genotypes by performing a genome-wide association study with an incomplete diallel cross population. Twenty-three major marker-trait associations (MTAs) resistance to B. sorokiniana black point were identified, which could explain more than 11% of the phenotypic variations. They were located on 1B, 1D, 2B (2), 2D, 3A (2), 3B (2), 3D, 4A, 5A (2), 5B (2), 6B (3), 6D, 7A (2), and 7D (2), respectively. The average number of major MTAs in the 10 resistant lines was 20.2, whereas that in the susceptible lines was 9.8. All the major MTAs in the parents were detected in the F1 hybrids. Three PCR markers were developed for detecting two MTAs using two recombinant inbred line populations. These PCR markers linked to black point resistance and accessions with a larger number of resistance alleles can be used to improve wheat resistance by QTLs pyramiding via MAS. |
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| Keywords: | Black point Genome-wide association study (GWAS) |
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