Stallion Sperm Integrity After Centrifugation to Reduce Seminal Plasma Concentration and Cool Storage for 4 days |
| |
| Affiliation: | 1. Department of Veterinary Clinical Sciences, School of Veterinary Medicine, Louisiana State University, Baton Rouge;2. School of Animal and Veterinary Sciences, School of Medicine, The University of Adelaide, Adelaide, Australia;1. College of Veterinary Medicine and Animal Science, CES University, Medellín, Colombia;2. College of Sciences, School of Chemistry, Universidad Nacional de Colombia, Medellín, Colombia;3. Department of Animal Production, Universidad Nacional de Colombia, Medellín, Colombia;1. Department of Veterinary Clinical Medicine, College Veterinary Medicine, University of Illinois Urbana-Champaign, Urbana, IL;2. Department of Large Animal Medicine, College of Veterinary Medicine, University of Georgia, Athens, GA;3. Department of Clinical Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY;1. University of Helsinki, Faculty of Veterinary Medicine, Paroninkuja 20, 04920, Saarentaus, Finland;2. University of Helsinki, Faculty of Veterinary Medicine, PO Box 66, 00014, Helsinki, Finland;1. Unit of Infection Diseases, University of Extremadura, Caceres, Spain;2. Laboratory of Equine Reproduction and Equine Spermatology, University of Extremadura, Caceres, Spain;3. Animal Reproduction and Obstetrics, University of León, León, Spain;4. Faculty of Veterinary Medicine and Animal Sciences, SLU, Uppsala, Sweden |
| |
| Abstract: | The objective of the study was to investigate if reducing the seminal plasma of stallion extended semen by centrifugation once will suffice to maintain acceptable semen quality for insemination after 4 days of cool storage. Collected semen was extended to 25 × 106 sperm/mL and subjected to one of the following treatments: noncentrifuged (control), centrifuged for 10 minutes at 900 × g and 1800 × g. The supernatant was partially removed, and the sperm pellet, reconstituted and re-extended. It was then placed in a passive cooling device overnight and then transferred to a refrigerator for the remainder of the cooling period. At day 0, 2, and 4, total motility (TM), progressive motility (PM), and plasma (PLM) and acrosomal membrane integrity were assessed. Centrifuged groups had higher TM and PM at day 4 than the control group (P < .05). Likewise, centrifuged groups had higher intact PLM in day 4 (P < .05). A single centrifugation cycle to reduce seminal plasma concentration will suffice to preserve sperm integrity acceptable for an artificial insemination dose up to 4 days of cool storage. |
| |
| Keywords: | Stallion Centrifugation Sperm motility Plasma membrane and cool storage |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
