Advances in Holding and Cryopreservation of Equine Oocytes and Embryos |
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| Affiliation: | 1. Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Montreal, QC, Canada;2. Department of Veterinary Physiology and Pharmacology, College of Veterinary Medicine & Biomedical Sciences, Texas A&M University, College Station, 77843, TX, USA;1. Department of Veterinary Medical Sciences, University of Bologna, Ozzano Emilia, Bologna, Italy;2. National Institute of Artificial Insemination, University of Bologna, Cadriano, Bologna, Italy;1. School of Animal Sciences, Louisiana State University Agricultural Center, Baton Rouge, LA;2. Louisiana Center for Equine Reproduction, Opelousas, LA;3. Department of Veterinary Clinical Sciences, Louisiana State University School of Veterinary Medicine, Baton Rouge, LA;4. Bob R. Jones–Idlewild Research Station, Louisiana State University Agricultural Center, Clinton, LA;1. Department of Animal Medicine and Surgery, Faculty of Veterinary Medicine, Veterinary Reproduction Group, University of Cordoba, Cordoba, Spain;2. Departamento de Producción Animal, Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata, Buenos Aires, Argentina;3. Department of Genetics, Faculty of Veterinary Medicine, MERAGEM Group, University of Cordoba, Cordoba, Spain |
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| Abstract: | Methods for holding of oocytes and embryos during shipment as well as for their cryopreservation can greatly aid equine reproductive management. Oocytes can be held at room temperature overnight or at cooler temperatures for two nights without affecting maturation or embryo development after intracytoplasmic sperm injection. In contrast, methods for cryopreservation of equine oocytes that support high rates of embryo development have not yet been established. Equine embryos may be held overnight at temperatures from 5°C to 19°C without reduction in viability, but longer holding periods, or higher holding temperatures, may be detrimental. Small equine embryos (<300 μm), either in vivo derived or in vitro produced, can be slow frozen or vitrified successfully. In the last decade, methods have been developed to allow in vivo–derived expanded blastocysts, up to Day 8, to be vitrified successfully after blastocoele collapse. These methods of shipment and preservation allow mare owners in remote locations to have access to sophisticated assisted reproductive technologies. |
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| Keywords: | Equine Embryo Oocyte Holding Cryopreservation |
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