Influence of low soil nitrogen and phosphorus on gluten polymeric and monomeric protein distribution in two high quality spring wheat cultivars |
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| Affiliation: | 1. National Agronomic Institute of Tunisia, 43 Avenue Charles Nicolle, 1082 Tunis Mahrajène, Tunisia;2. High school of food industries, 58 Avenue Alain Savary, C Khadra, Tunis, Tunisia;1. National Technique Innovation Center for Regional Wheat Production, Key Laboratory of Crop Physiology and Ecology in Southern China, Ministry of Agriculture, National Engineering and Technology Center for Information Agriculture, Nanjing Agricultural University, PR China;2. Department of Molecular Biology and Genetics, Section of Crop Genetics and Biotechnology, Aarhus University, Forsøgsvej 1, 4200 Slagelse, Denmark;3. Department of Agroecology, Aarhus University, Forsøgsvej 1, 4200 Slagelse, Denmark;1. INIA La Estanzuela, Colonia, CP 70000, Uruguay;2. University of Agricultural Sciences, Department of Plant Breeding, PO Box 101, SE-230 53, Alnarp, Sweden;1. College of Water Resources and Architectural Engineering, Northwest A&F University, Yangling 712100, Shaanxi, China;2. Jiyang College of Zhejiang A & F University, Zhuji 311800, Zhejiang, China;3. Institute of Soil and Water Conservation, Chinese Academy of Sciences and Ministry of Water Resources, Yangling 712100, Shaanxi, China;4. Institute of Water Saving Agriculture in Arid Areas of China, Northwest A&F University, Yangling 712100, Shaanxi, China |
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| Abstract: | The effect of low levels of nitrogen, phosphorus and a combination of the two on the distribution of polymeric and monomeric proteins in two high quality spring bread wheat cultivars was investigated for two consecutive seasons. Size exclusion-high performance liquid chromatography (SE-HPLC) was used to determine the quantity and relationships of monomeric and polymeric proteins, and their relationship with flour protein content (FPC) and SDS sedimentation volume (SDSS). The low nitrogen and combined low nitrogen and low phosphorus treatments had a much larger effect on the protein fractions than the low phosphorus treatment alone. The SDS-soluble large monomeric protein fraction and the percentage SDS-insoluble monomeric proteins, were significantly increased under low nitrogen and a combination of low nitrogen and low phosphorus treatments. The percentage SDS-insoluble large and total polymeric proteins was significantly reduced under low nitrogen and a combination of low nitrogen and phosphorus treatments. The SDS-soluble and -insoluble small polymeric proteins were significantly increased under both low nitrogen and a combination of low nitrogen and low phosphorus treatments. The low nitrogen treatment consistently caused the lowest FPC and SDSS values. Under low nitrogen conditions, there was a significant positive correlation between the SDS-soluble gliadins and SDSS, and FPC. |
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| Keywords: | Glutenin Gliadin Protein content Fertilizer |
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