| 抗稻瘟病链霉菌JK-1基因组BAC文库构建 |
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| 引用本文： | 张学江,曾凡松,杨立军,龚双军.抗稻瘟病链霉菌JK-1基因组BAC文库构建[J].安徽农学通报,2014(24):17-18. |
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| 作者姓名： | 张学江 曾凡松 杨立军 龚双军 |
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| 作者单位： | 湖北省农业科学院植保土肥研究所/农业部华中作用有害生物综合治理重点实验室/农作物重大病虫草害防控湖北省重点实验室,湖北武汉,430064 |
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| 基金项目： | 湖北省农业科技创新中心资助项目(2011-620-003-03-04)。 |
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| 摘 要： | 实验以抗稻瘟病链霉菌JK-1的基因组DNA为材料构建了其BAC（bacterial artificial chromosome）文库。该文库共有3456个克隆，插入片段在40-100kb，平均插入片段55kb，空载率低于5%，覆盖了链霉菌基因组约17.3倍。抗稻瘟病链霉菌JK-1基因组BAC文库的构建，为进一步研究基因组结构及其功能基因的利用等奠定了基础。
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| 关 键 词： | 链霉菌 BAC文库 基因组 |
Construction of the Bacterial Artificial Chromosome Library of Streptomyces JK-1 Resistance to Magnaportheoryzae |
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| Institution: | Zhang Xuejiang et al. (Institute of Plant Protection and Soil Science,Hubei Academy of Agricultural Sciences/Key Laboratory of Integrated Pest Management on Crops in Central China, Ministry of Agricuhure/Hubei Key Laboratory of Crop Diseases,Insect Pests and Weeds Control, Wuhan 430064, China) |
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| Abstract: | A genomic bacterial artificial chromosome（BAC）library was constructed using nuclear DNA of Streptomyces JK-1 resistance to Magnaporthe oryzae. The library collected 3 456 recombinant clones carry？ing inserts with sizes ranging from 40kb to 100kb. The average insert size was about 55kb with empty-vec？tor rate less than 5%. This BAC library represented 17.3 equivalents of Streptomyces genome. The BAC li？brary will help further genome and fucntional gene research. |
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| Keywords: | Streptomyces BAC library Genome |
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