燕麦D基因组特异标记开发 |
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引用本文: | 翟志文,柴国师,候莎莎,剌士潇,靳艳婷,贾举庆. 燕麦D基因组特异标记开发[J]. 农学学报, 2015, 5(10): 88-92. DOI: 10.11923/j.issn.2095-4050.cjas15050012 |
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作者姓名: | 翟志文 柴国师 候莎莎 剌士潇 靳艳婷 贾举庆 |
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作者单位: | 山西农业大学农学院,电子科技大学生命科学技术学院,山西农业大学农学院,山西农业大学农学院,山西农业大学农学院,山西农业大学农学院 |
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基金项目: | 山西省普通高等学校大学生创新创业训练项目“野生燕麦基因组特异标记的开发与应用”(2012075);山西农业大学大学生科技创新项目“野生燕麦基因组特异标记的开发与应用”(10-004);山西农业大学引进人才科研启动项目“燕麦基因组中反转录转座子的分离与应用”(XB2010009)。 |
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摘 要: | 为了提高燕麦分子育种的效率,旨在开发燕麦D基因组特异分子标记。先利用300 条10 碱基随机引物对9 种燕麦种材料进行随机多态性扩增(random amplified polymorphic DNA,RAPD),扩增出燕麦D基因组特异性条带,命名为OPAB011287,经Blast 比对后发现该条带为一反转座子重复序列,根据此条带序列设计特异性引物,建立更简便稳定的SCAR标记。结果发现该SCAR标记可以准确特异的检测到9 种试验材料中含有D基因组的燕麦品种,结论表明该标记可用于燕麦分子标记辅助育种,有效服务于燕麦优良品种培育。
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关 键 词: | 花生 花生 品种 光氧化 抗性评价 |
收稿时间: | 2015-05-12 |
修稿时间: | 2015-05-26 |
Development of D Ggenome-specific Marker in Oat |
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Abstract: | The research aims to improve the efficiency of molecular breeding and develop D- Genome-specific markers in oat. Firstly, random amplified polymorphic DNA (RAPD) analysis was performed on 9 oat varieties with 300 random 10-based primers. A repetitive sequence of 1287 bp, named OPAB011287 was identified in the D genome of oat. BLAST search revealed that the sequence of OPAB011287 was highly homologous to a part of a retrotransposon. Based on the sequence of OPAB011287, a pair of sequence- characterized amplified region primers (SCAR) was designed which was more convenient and stable. And the results showed that SCAR marker was specific to target oat with D genome, which provided a powerful tool for oat marker assisted breeding and the better service for the breeding of oat. |
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Keywords: | Oat D Genome Repetitive Sequence Molecular Marker |
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