<Emphasis Type="Italic">De novo</Emphasis> assembly of potential linear artificial chromosome constructs capped with expansive telomeric repeats |
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| Authors: | Li Lin Dal-Hoe Koo Wenli Zhang Joseph St Peter Jiming Jiang |
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| Institution: | (1) Department of Horticulture, University of Wisconsin-Madison, Madison, WI 53706, USA |
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| Abstract: | Background Artificial chromosomes (ACs) are a promising next-generation vector for genetic engineering. The most common methods for developing
AC constructs are to clone and combine centromeric DNA and telomeric DNA fragments into a single large DNA construct. The
AC constructs developed from such methods will contain very short telomeric DNA fragments because telomeric repeats can not
be stably maintained in Escherichia coli. |
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