| 外源激素对肥皂草丛生芽诱导的影响 |
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| 引用本文： | 王培军.外源激素对肥皂草丛生芽诱导的影响[J].辽宁农业职业技术学院学报,2012,14(4):6-7,19. |
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| 作者姓名： | 王培军 |
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| 作者单位： | 太原大学外语师范学院,山西太原,030012 |
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| 摘 要： | 本研究以MS为基本培养基，采用肥皂草的叶片为外植体进行组织培养，通过研究不同配比模式的外源激素，探索外源激素对肥皂草丛生芽诱导的关键影响因子，同时有效解决了诱导过程中培养基污染和外植体褐变问题。结果表明，当肥皂草丛生芽诱导培养基为MS＋6一BA3.0 mg／L＋NA A0．05mg/L时，丛生芽诱导倍数达到4．53，效果最好。2,4-D诱导叶片生成丛生芽的效果不显著。
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| 关 键 词： | 外源激素 肥皂草 外植体 丛生芽 |
Effects of Growth Conditioner on Forming buds of Saponaria officinalis L. |
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| WANG Peijun.Effects of Growth Conditioner on Forming buds of Saponaria officinalis L.[J].Journal of Liaoning Agricultural Vocation-Technical College,2012,14(4):6-7,19. |
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| Authors: | WANG Peijun |
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| Institution: | WANG Peijun(Foreign Language Nomal Colege of Taiyuan University,Taiyuan 030012,China) |
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| Abstract: | The leaf of Saponaria officinalis L.was used explant to establish the technique system of tissue culture.A study was cultured on the MS added with different concentration of cytokinin and the auxin,we wanted to find out the best medium composition.The results indicated that the best callus initiation medium was MS+6-BA3.0 mg/L+NAA0.05 mg/L propagation multiple amounts to 4.53.effect of 2,4-D on regenerate shoots initiation was unapparent. |
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| Keywords: | Growth conditioner Saponaria oKicinalis L regenerate shoot Explant |
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