| 运用RAPD对核桃属种间亲缘关系的研究 |
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| 引用本文： | 吴燕民,裴东,奚声珂,李嘉瑞. 运用RAPD对核桃属种间亲缘关系的研究[J]. 园艺学报, 2000, 27(1): 17-19 |
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| 作者姓名： | 吴燕民 裴东 奚声珂 李嘉瑞 |
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| 作者单位： | 1. 中国林业科学研究院林业研究所,北京,100091
2. 西北农业大学园艺系,杨凌,712100 |
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| 基金项目： | 林业部林木培育重点开放实验室资助项目 |
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| 摘 要： | 用随机扩增多态性ＤＮＡ（ＲＡＰＤ）技术对核桃属内９个种及近缘属的２个种进行基因组ＤＮＡ多态性分析，共选用２０个１０ｂｐ随机引物扩增出３１１个ＤＮＡ片段，其中铁核桃Ｊ．ｓｉｇｉｌｌａｔａ，甘肃枫杨Ｐ．ｍａｃｒｏｐｔｅｒａ显示出自身特征性标记，利用这些片段进行遗传关系分析，根据ＵＰＧＭＡ方法构建聚类树状图。研究结果表明：铁核桃为核桃属中一个独立种；核桃属内组间和种间亲缘关系与经典分类学的结果完全一致；
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| 关 键 词： | 核桃属 RAPD 亲缘关系 分类 种质资源 开发利用 |
| 修稿时间： | 1999-06-03 |
A Study on the Genetic Relationship Among species in Juglans L.Using RAPD Markers |
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| Wu Yanmin,Pei Dong,Xi Shengke,Li Jiarui. A Study on the Genetic Relationship Among species in Juglans L.Using RAPD Markers[J]. Acta Horticulturae Sinica, 2000, 27(1): 17-19 |
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| Authors: | Wu Yanmin Pei Dong Xi Shengke Li Jiarui |
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| Affiliation: | Department of Neurobiology, Third Military Medical University, Chongqing 400038, China |
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| Abstract: | Random amplified polymorphic DNA(RAPD)technique was used to analyze the genetic relationship of 11 species of Juglans and its relatives.Twenty decanucleotide arbitrary primers were selectol and 311 DNA fragments were generated,these fragments were used in the analysis of the relationship of the 11 species.The dendrogram was constructed by using UPGMA methods based on their genetic distance,giving the taxonomic position of these species.Specific markers were found in J.sigillata ,this indicates that it is a independent species.The availability of RAPD in the analysis of the genetic relationship at the genus level needs further study. |
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| Keywords: | Juglans L. RAPD Relationship |
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