Genetic diversity and population structure of four Iranian alfalfa populations revealed by sequence-related amplified polymorphism (SRAP) markers |
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| Authors: | Majid Talebi Zahra Hajiahmadi Mehdi Rahimmalek |
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| Institution: | (1) Department of Agronomy and Plant Breeding, Faculty of Agriculture, Urmia University, Urmia, Iran;(2) Department of Agricultural Biotechnology, Institute of Biotechnology, Urmia University, Urmia, Iran;(3) Department of Agronomy and Plant Breeding, Faculty of Agriculture, Islamic Azad University of Tabriz, Tabriz, Iran |
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| Abstract: | In the present study, genetic diversity of 48 individual plants from four Iranian cultivated populations of alfalfa (Medicago sativa L.) was evaluated using sequence-related amplified polymorphism (SRAP) markers. Fourteen SRAP primer combinations produced
193 fragments of which 95 were polymorphic. The number of polymorphic fragments detected per primer combination ranged from
3 to 10 bands with an average of 6.78 bands. Average polymorphic information content (PIC) was 0.343 for all primer combinations.
Although the AMOVA (analysis of molecular variance) results showed a significant difference in the genetic diversity among
the populations (P < 0.0001), the genetic variation mainly caused by the variation of intra population accounted for 93.17%
of the total genetic variation. Unweighted pair-group method arithmetic average (UPGMA) analysis of the marker data clearly
separated the populations of subtropical (Yazdi) and semi-cold (Hamadani and Nikshahri) as well as Kodi, an improved population.
It can be concluded that SRAP markers are useful for studying diversity and relationships among and within alfalfa populations. |
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