| 氟烷基因在三江自猪猪群、迪卡配套系猪B系、亚洲野猪中的分布及其对肉质的影响 |
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| 引用本文： | 耿忠诚,袁子国,刘德贵,靳锐,陈建华,刘丽丽,迟兆江. 氟烷基因在三江自猪猪群、迪卡配套系猪B系、亚洲野猪中的分布及其对肉质的影响[J]. 黑龙江畜牧兽医, 2006, 0(11): 19-22 |
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| 作者姓名： | 耿忠诚 袁子国 刘德贵 靳锐 陈建华 刘丽丽 迟兆江 |
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| 作者单位： | 黑龙江八一农垦大学 动物科技学院，黑龙江八一农垦大学 动物科技学院，北京养猪育种中心，黑龙江八一农垦大学 动物科技学院，黑龙江八一农垦大学 动物科技学院，黑龙江八一农垦大学 动物科技学院，黑龙江省农垦总局 科技局 黑龙江 大庆163319，黑龙江 大庆163319，北京100085，黑龙江 大庆163319，黑龙江 大庆163319，黑龙江 大庆163319，黑龙江 哈尔滨150020 |
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| 基金项目： | 黑龙江省科技攻关项目(GB01B105-01) |
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| 摘 要： | 试验随机选取138头迪卡配套系猪B系、216头三江白猪和192头亚洲野猪提取基因组DNA进行聚合酶链式反应,得到RYR_1基因特异片段,经过双酶切鉴定,测得Hal~N基因频率分别为87．31%、92．13%、92．97%；Hal~n的基因频率分别为12．69%、7．87%、7．03%。然后对3种氟烷基因型的猪进行肉质鉴定。
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| 关 键 词： | 三江白猪 聚合酶链反应 氟烷基因 肉质 |
| 文章编号： | 1004-7034（2006）11-0019-04 |
| 收稿时间： | 2006-04-11 |
| 修稿时间： | 2006-04-11 |
Distributing probability of three genotypes of RYR1 of Sanjiang White pigs |
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| GENG Zhong- cheng , YUAN Zi - guo , LIU De - gui, et al. Distributing probability of three genotypes of RYR1 of Sanjiang White pigs[J]. Heilongjiang Animal Science And veterinary Medicine, 2006, 0(11): 19-22 |
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| Authors: | GENG Zhong- cheng YUAN Zi - guo LIU De - gui et al |
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| Affiliation: | 1. Heilongjiang August First Land Reclamation Unversity , Daqing 163319,China;2. The Center of Breeding Pigs of Beijing,Beijing 100085 ,China |
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| Abstract: | 138 DIKERS,216 white pigs,198 Sus scrofa were selected randomly,selecting blood samples from Sanjang White pigs,the DNA were distilled in blood anti purified,by PCR amplified and enzyme digestied,the genotypes of RYRI was analysised.The results indicated that:after conditioning optimized by PCR,a fragment of 659 bp was amplified by PCR,the fragment was digested by Hha l and Hgih I,three genotypes of Hal~NHal~N,Hal~N Hal~n,Hal~n Hal~n were gained.The frequency of HalN genotype among three species was 87.31%,92.13%,92.97% in turn.The frequency of Haln genotype was 12.69%, 7.87%,7.030%. (009) |
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| Keywords: | Halothane gene Sanjiang White pigs gene frequency |
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