HPLC测定不同蜜源中10-HDA的含量

建立了二极管阵列检测器测定不同蜜源新鲜蜂王浆中10-羟基-2-癸烯酸(10-HDA)的反相高效液相色谱分析方法,色谱条件为色谱柱Nucleodur C18(4.6 mm×250 mm,5μm),流动相甲醇—水—磷酸(55∶45∶2,V/V),流速1.0 ml/min,检测波长212 nm,柱温25℃。10-HDA在1.384~12.456μg范围内与峰面积呈良好线性关系(r=0.999 9),加样平均回收率为99.6%。该方法简便、准确、重现性好、线性范围宽,适用于评价蜂王浆及其产品的质量。

Determination of 10-HDA in Different Royal Jelly by HPLC

Adetermination method of 10-hydroxy-2-decenoic acid in fresh royal jelly from different honey sources by reversed-phase high performance liquid chromtograph was established and at the time the purity of 10-HDA peak was detected by means of Photodiode Array Detector(PAD).The chromatographic column,Nucleodur C18(4.6 mm×250 mm,5 μm),methanol-water-phosphoric acid(55∶45∶2,V/V) mobile phase with 1.0 ml/min flow rate,the detected wavelength(212 nm)and the column temperature(25 ℃) were adopted.The calibration curve of 10-HDA was linear under the content of 1.384～12.456 μg,the correlation coefficient was over 0.999 9.The average recovery of 10-HDA was 99.6 %.The method was simple,accurate,reproducible and had wider linear range.The method can be adapted to evaluate the quality of royal jelly and preparation.