| 罗氏沼虾白斑综合征病毒囊膜蛋白VP28基因的克隆及分析 |
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| 引用本文： | 王晓兰,贡成良,薛宇醒,曹广力,魏育红,陈辉,许雅香,张伟明,薛仁宇. 罗氏沼虾白斑综合征病毒囊膜蛋白VP28基因的克隆及分析[J]. 淡水渔业, 2007, 37(3): 12-16 |
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| 作者姓名： | 王晓兰 贡成良 薛宇醒 曹广力 魏育红 陈辉 许雅香 张伟明 薛仁宇 |
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| 作者单位： | 苏州大学生命科学学院,江苏苏州,215123;江苏省水生动物疫病控制中心,南京,210036;苏州市水产研究所有限公司,江苏苏州,215107 |
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| 基金项目： | 苏州市攻关课题，国家重点基础研究发展计划(973计划) |
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| 摘 要： | 根据已公布的罗氏沼虾白斑综合征病毒(WSSV)囊膜蛋白VP28基因序列设计一对特异性引物,从疑似患白斑病毒病的罗氏沼虾(Macrobrachium rosenbergii)中提取总DNA,并以此为模板,经PCR扩增、克隆并测序后将该片段通过GenBank比对,证实为WSSV的VP28基因;与20个已公布的WSSV VP28进行同源性比较,结果显示:从中国对虾、斑节对虾、南美白对虾、日本对虾、波纹龙虾提取的病毒株聚为一类,印度对虾WSSV VP28为另一类,罗氏沼虾WSSV VP28又单独为一类。根据测序结果推测VP28蛋白的二级结构在氨基酸的7～29区间可能为跨膜螺旋区,且该区域高度保守。
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| 关 键 词： | VP28基因 克隆 序列分析 蛋白质二级结构 |
| 文章编号： | 1000-6907-（2007）03-0012-05 |
| 修稿时间： | 2006-11-08 |
Gene Clone and Analysis of Envelope Protein VP28 of White Spot Syndrome Virus of Macrobrachium rosenbergii |
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| WANG Xiao-lan,GONG Cheng-liang,XUE Yu-xing,CAO Guang-li,WEI Yu-hong,CHEN Hui,XU Ya-xiang,ZHANG Wei-ming,XUE Ren-yu. Gene Clone and Analysis of Envelope Protein VP28 of White Spot Syndrome Virus of Macrobrachium rosenbergii[J]. Freshwater Fisheries, 2007, 37(3): 12-16 |
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| Authors: | WANG Xiao-lan GONG Cheng-liang XUE Yu-xing CAO Guang-li WEI Yu-hong CHEN Hui XU Ya-xiang ZHANG Wei-ming XUE Ren-yu |
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| Affiliation: | 1. Life Sciences College, Soochow University, Suzhou, Jiangsu 215123; 2 . Aquatic Animals Disease Control Centers of Jiangsu Province, Nanjing 210036; 3. Aquatic Research Institute Ltd. of Suzhou, Suzhou, Jiangsu 215107 |
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| Abstract: | A special pair of primers was designed based on white spot syndrome virus(WSSV)released from GenBank to amplify a fragment with total DNA template from Macrobrachium rosenbergii which was suspicious of being infected with WSSV.Then the fragment was cloned and sequenced.Blast analysis indicates the fragment belongs to VP28 of WSSV.Compared the WSSV VP28 gene from M.rosenbergii with twenty published ones on GenBank and devised the dendrogram.The result showed that the virus from Penaeus Chinensis,P.monodon,P.setiferus,P.japonicus,and P.homarus were congeneric.The one from P.indicus was another genus,and the one from M.rosenbergii was different.The membrane helix from 7 to 29 was predicted according to the amino acid sequence of VP28,and it was highly reserved. |
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| Keywords: | VP28 gene clone sequence analysis second structure of protein |
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