| 利用SSH技术分离甘蓝抗黑腐病相关基因的研究 |
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| 引用本文： | 朱妍,王超.利用SSH技术分离甘蓝抗黑腐病相关基因的研究[J].中国蔬菜,2010,1(10):20-24. |
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| 作者姓名： | 朱妍 王超 |
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| 作者单位： | 东北农业大学园艺学院，黑龙江哈尔滨 150030 |
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| 基金项目： | 国家“863”计划(2006AA100108-4-9) |
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| 摘 要： | 采用抑制差减杂交(SSH)技术,构建甘蓝抗病品种A21经黑腐病菌诱导6、8、12、24h后的混合SSH文库。经反向Northern筛选,共获得87条高质量ESTs。通过NCBI中BLAST数据库比对分析发现,83个ESTs与已知蛋白或基因具有不同程度的同源性。
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| 关 键 词： | 甘蓝 黑腐病 SSH |
| 收稿时间： | 2010-01-04; |
Studies on Isolation of Resistance Related-genes to Black Rot by Suppression Subtractive Hybridization in Brassica oleracea |
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| ZHU Yan,WANG Chao.Studies on Isolation of Resistance Related-genes to Black Rot by Suppression Subtractive Hybridization in Brassica oleracea[J].China Vegetables,2010,1(10):20-24. |
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| Authors: | ZHU Yan WANG Chao |
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| Institution: | College of Horticulture, Northeast Agricultural University, Harbin 150030, Heilongjiang, China |
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| Abstract: | In this research,suppression subtractive hybridization(SSH)was adopted to construct a mixed SSH library by inducing black rot to disease resistant cabbage(Brassica oleracea L.)variety A21 for 6h,8h,12h and 24h.Through reverse northern screening,87 ESTs with high quality were obtained.After conducting comparative analysis with BLAST data bank in NCBI,83 of the ESTs were found to have homology by different degrees with known protein or genes. |
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| Keywords: | Brassica oleracea Black rot SSH |
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