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Extraction of soil nitrogen by chloroform fumigation – A new index for the evaluation of soil nitrogen supply
Authors:Wantai Yu  Philip C. Brookes  Qiang Ma  Hua Zhou  Yonggang Xu  Shanmin Shen
Affiliation:aInstitute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, PR China;bSustainable Soils and Grassland Systems Department, Rothamsted Research, Harpenden, Herts. AL5 2JQ, UK1
Abstract:The N extracted after chloroform (CHCl3) fumigation was determined as a possible index of soil N supply to plants. The relationships between extractable N following fumigation and reference indices such as total N, alkali-hydrolyzable N, N released by the Stanford short-term incubation method, and the N extracted by KCl and by CaCl2, were measured in nine soils of differing soil N supply capacity. A highly significant correlation was achieved between the extractable N released by fumigation and the N released by the Stanford method, i.e. a short-term aerobic incubation (r = 0.87). Similarly, the correlation between extractable N by fumigation and the N uptake by ryegrass was highly statistically significant (r = 0.93). Using the N extracted following fumigation has the advantage that laboratory results are available in two days and are both reproducible and of high precision. Therefore, the N extracted following fumigation is a valid, timesaving and precise index of soil N supply capacity.
Keywords:N supply   N extracted by chloroform fumigation   N uptake
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