适于制作人工种子的一品红体细胞胚的培养

 MS + 2,4-D 2.0 mg·L ^{- 1} + 6-BA 0.5 mg·L ^{- 1}固体培养基较适合一品红苞片诱导愈伤组织。MS+ 2,4-D 1.0 mg·L ^{- 1} + 6-BA 0.5 mg·L ^{- 1} +水解酪蛋白80 mg·L ^{- 1}固体培养基上体细胞胚分化率最高。活性炭、MS培养基和悬浮振荡培养有利于高频率体细胞胚发生。球形、心形及梨形胚及时转入添加NAA 0.2 mg·L ^{- 1}、6-BA 0.5 mg·L ^{- 1}及水解酪蛋白80 mg·L ^{- 1}的MS液体培养基中, 有利于高质量体细胞胚发生。子叶期胚及时转入添加ABA 0.5 mg·L ^{- 1}的上述培养基中, 会提高正常体细胞胚的百分率。用高质量(子叶期) 的一品红体细胞胚包埋制作人工种子, 在无菌条件下萌发率为58.1%。

Studies on High Quality Somatic Embryogenesis Applied to Artificial Seed of Euphorbia pulcherrima Willd

MS agarmedium supp lemented with 2,4-D 2.0 mg·L^-1 and 6-BA 0.5 mg·L^-1 was proved to be the best for inducing callus from the leaves of Euphorbia pulcherrima Willd. It was suitable for somatic embryogenesis when the concentration of 2,4-D was reduced to 1.0 mg·L^-1. Activated carbon, MSmedium and suspension culture were suitable for high frequency somatic embryogenesis. If globular embryoids, heart-shaped embryoids and pear 6-BA shaped embryoidswere transferred intoMSmedium supplemented with NAA0.2 mg·L ^-1, 6-BA 0.5 mg·L ^-1 and casein hydrolyzed 80 mg·L ^-1 , the quality of somatic embryos would be improved. If cotyleden embryoids were transferred in time into a forementioned medium added on ABA 0.5 mg·L ^-1 , the rate of normal somatic embryogenesis would be increased.