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ABA和AgNO3对小麦幼胚愈伤组织诱导和分化的影响
引用本文:陈军营,文付喜,何盛莲,陈新建,许海霞,程西永.ABA和AgNO3对小麦幼胚愈伤组织诱导和分化的影响[J].麦类作物学报,2006,26(2):46-48.
作者姓名:陈军营  文付喜  何盛莲  陈新建  许海霞  程西永
作者单位:河南农业大学农学院,郑州,450002
基金项目:河南省高校杰出科研创新人才工程项目
摘    要:为解决河南小麦生产上三个主导品种(豫麦18号、豫麦49号、兰考906)生物技术改良的技术瓶颈问题。研究了不同浓度的ABA和AgNO3处理对上述三个小麦品种幼胚愈伤组织诱导和分化特性的影响。结果表明。添加0.1mg/LABA的诱导培养基以及在添加0.1mg/l ABA的基础上再添加2.5mg/L或5.0mg/LAgNO3的诱导培养基对幼胚愈伤组织形成和分化最为有利,但不同基因型在相同的处理下幼胚愈伤组织诱导率和分化率仍存在较大的差异。

关 键 词:小麦幼胚  愈伤组织
文章编号:1009-1041(2006)02-0046-03
收稿时间:2005-09-21
修稿时间:2005-11-20

Effect of ABA and AgNO3 on Calli Induction and Differentiation of Immature Wheat Embryo
CHEN Jun-ying,WEN Fu-xi,HE Sheng-lian,CHEN Xin-jian,XU Hai-xi,CHENG Xi-yong.Effect of ABA and AgNO3 on Calli Induction and Differentiation of Immature Wheat Embryo[J].Journal of Triticeae Crops,2006,26(2):46-48.
Authors:CHEN Jun-ying  WEN Fu-xi  HE Sheng-lian  CHEN Xin-jian  XU Hai-xi  CHENG Xi-yong
Institution:Agronomy College, Henan Agricultural University, Zhengzhou, Henan 450002, China
Abstract:In order to overcome the technique obstacle of genetic improvement in some local main wheat cultivars,effects of different factors including abscisic acid(ABA) and AgNO_3 on calli induction and differentiation of immature embryos derived from three wheat cultivars(Yumai 18,Yumai 49 and Lankao 906) were studied.The result showed that callus formation and differentiation of immature embryos were apparently improved on Induction medium supplement with 0.1 mg/L ABA and 2.5 mg/L or 5.0 mg/L AgNO_3.There were significant differences among the different genotypes.
Keywords:ABA  AgNO3
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