Decomposition of atmospheric hydrogen by soil microorganisms and soil enzymes |
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Authors: | Ralf Conrad Wolfgang Seiler |
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Affiliation: | Max-Planck-Institut für Chemie, Saarstr. 23, D-6500 Mainz, West Germany |
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Abstract: | The decomposition of atmospheric hydrogen in different types of soil was measured. The decomposition of H2 was apparently a first-order reaction. H2 decomposition activity was proportional to the amount of soil with maximum activities at soil water contents of approx. 6–11% (w/w). The activity was lower under anaerobic conditions, but was constant between 1–20% O2. It was destroyed by autoclaving and was partially inactivated by fumigation with NH3, CHC13 or acetone, by u.v. irradiation and by treatment with NaCN or NaN3, indicating that biological processes in the soil were responsible for the observed H2 decomposition. Treatment of soil with toluene or CHCl3 caused only a partial inactivation. Incubation of soil in the presence of streptomycin or actidione reduced H2 decomposition by less than 50%, whereas CO consumption was abolished. The H2 decomposition rates showed H2 saturation curves with apparent Michaelis-Menten kinetics. Cooperative effects were not observed. Vmax was reached at approx. 200 μl1?1. The Km values for H2 were in the range of 30μl 1?1, but increased to higher values, when the soil had been pretreated with high H2 mixing ratios. Apparently, the observed H2 decomposition by soil is not only due to the activity of viable microorganisms, but soil enzymes as well. |
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