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云南魔芋新病害—疫病病原菌的鉴定
引用本文:王瑞仙, 邵梅, 于德才, 等. 魔芋疫病烟草疫霉菌(Phytophthora nicotianae)的致病性研究[J]. 云南农业大学学报(自然科学), 2018, 33(2): 213-217. DOI: 10.12101/j.issn.1004-390X(n).201709037
作者姓名:王瑞仙  邵梅  于德才  宋双伟  卢俊  徐雅  张红骥
作者单位:1.云南农业大学 植物保护学院,农业生物多样性与病虫害控制教育部重点实验室,云南 昆明 650201;2.云南省富源魔芋研究所,云南 富源 655400
基金项目:国家“973”项目(2011CB100400)
摘    要:ttttt目的ttttt明确烟草疫霉对魔芋的致病性,为其引起的魔芋疫病的防治提供理论基础。ttttttttttttt方法ttttt对分离鉴定的5株烟草疫霉菌,在温室和实验室条件下利用植物活体和离体接种法进行致病性鉴定。ttttttttttttt结果ttttt5株疫霉菌对魔芋均表现出相似的强致病性,发病率达到100%。实验室致病性鉴定表明:疫霉菌株Ps1对叶片(病情指数98.33)和根部(病情指数94.07)致病力显著高于球茎(病情指数78.33)和茎秆(病情指数71.11);在辣椒果实、马铃薯叶片、大豆和烟草茎基部分别接种Ps1菌株,不同寄主均出现明显病斑,其中疫霉菌对马铃薯的致病性(病情指数60.67)最强,对大豆的致病性最弱(病情指数32.59)。ttttttttttttt结论ttttt疫霉菌不仅对魔芋的不同部位有较强的致病性,还可侵染其他植物。tttt

关 键 词:魔芋  软腐病  分子鉴定  烟草疫霉菌  致病性
收稿时间:2017-09-19
修稿时间:2017-11-06

First report of stem rot or konjac caused by Phytophthora nicotianae in China
Ruixian WANG, Mei SHAO, Decai YU, et al. Pathogenicity Identification of Phytophthora nicotianae Causing Konjac Disease in Yunnan, China[J]. JOURNAL OF YUNNAN AGRICULTURAL UNIVERSITY(Natural Science), 2018, 33(2): 213-217. DOI: 10.12101/j.issn.1004-390X(n).201709037
Authors:Ruixian WANG  Mei SHAO  Decai YU  Shuangwei SONG  Jun LU  Ya XU  Hongji ZHANG
Affiliation:1.Key Laboratory for Agro-Biodiversity and Pest Control of Ministry of Education, College of Plant Protection, Yunnan Agricultural University, Kunming 650201, China;2.Institute of Konjac of Fuyuan County of Yunnan Province, Fuyuan 655400, China
Abstract:tttttPurposetttttIn order to provide the theoretical foundation for prevention and control of konjac blight causing by Phytophthora nicotianae, the pathogenicity of Phytophthora should be determined.ttttttttttttttttttMethodtttttFive Phytophthora strains were tested using inoculation in vitro and in vivo method in laboratory and greenhouse conditions.ttttttttttttttttttResultstttttFive strains had the similar pathogenicity to konjac, and the disease incidence reach 100%. The pathogenicity of P. nicotianae to leaves (disease index 98.33) and root (disease index 94.07) were significantly higher than seeds bulbs (disease index 78.33) and stem (disease index 71.11) in the laboratory. Besides, red pepper, soybean root, potato leaf and tobacco stem base were inoculated with Ps1 strains respectively, clear spots were found in different hosts, and P. nicotianae was the strongest pathogenic to potato (disease index 60.67) and the weakest pathogenic to soybean (disease index 32.59).ttttttttttttttttttConclusionstttttThe P. nicotianae can not only infect konjac, but also infect other host.ttttttttt
Keywords:konjac  soft rot  molecular identification  Phytophthora nicotianae  pathogenicity
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