几种真菌DNA提取方法的比较

ttttt目的ttttt确定在广东省广州市中山大学校园采集到的1份大型真菌标本的分类地位。ttttttttttttt方法ttttt运用分子系统学分析(构建基于rDNA ITS序列系统发育树)和传统大型真菌形态学鉴定相结合的方法，对采集到的标本进行了鉴定和描述。形态学鉴定包括宏观和微观两方面，宏观特征主要依据采集标本时在野外拍摄的新鲜子实体图片和相关的测量描述记录，微观特征则根据干标本进行徒手切片及显微观察和测量(包括对孢子形态和纹饰的扫描电镜观测)。ttttttttttttt结果ttttt采集到的标本与锐棘秃马勃(Calvatia holothurioides)的ITS序列相似性高达99%，两者在构建的系统发育树中聚成一枝，且宏观和微观形态特征也基本一致。tttttttttttttttttt结论ttttt根据分子系统学和形态学鉴定，采集到的标本确定为锐棘秃马勃(C. holothurioides)，为中国新记录种。ttttttttt

Arctic Gasteromycetes II. Calvatia in Greenland,Svalbard and Iceland

tttttPurposetttttTo identify a macro-fungi specimen collected in the campus of Sun Yat-sen University in Guangzhou, Guangdong Province.tttttttttttttMethodstttttBoth phylogenetic analysis (building NJ tree based on rDNA ITS sequences) and morphological study were applied to identification of the specimen. Morphology included macro- and micro-morphological features, the former was mainly based on fresh fruiting bodies and field notes, and the latter was obtained from microscopic observation and measurement (including that of spore size and ornamentation by SEM) of hand-sectioned dry specimen.tttttttttttttResultstttttSequence similarity of the examined specimen with Calvatia holothurioides is 99%, they clustered together with strong support in the phylogram of NJ tree and shared consistent morphological characteristics.ttttttttttttttttttConclusiontttttThe collected specimen was identified as C. holothurioides based on both phylogenetic and morphological evidence, and it is recognized as a new record of China.ttttttttt