Purification and characterization of glutathione peroxidase 1 in the red muscle of Pacific bluefin tuna Thunnus orientalis |
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Authors: | Yamashita Yumiko Yabu Takeshi Touhata Ken Yamashita Michiaki |
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Institution: | (1) National Research Institute of Fisheries Science, Kanazawa, Yokohama Kanagawa, 236-8648, Japan; |
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Abstract: | Glutathione peroxidase GPx1 was purified from the red muscle of Pacific bluefin tuna Thunnus orientalis and its enzymatic properties were characterized. The enzyme was optimally active at pH 7.4 with a specific activity for hydrogen
peroxide and a K
m of 6.7 μM. cDNA was also isolated and it contained a predicted open reading frame (ORF) encoding a 188 amino acid protein.
The phylogenetic tree shows that fish including Pacific bluefin tuna, pufferfish, and zebrafish, but not mammals, possess
two genetically distinct types of GPx1, i.e., GPx1a and GPx1b. The purified enzyme was classified as a fish GPx-1b enzyme
on the basis of the phylogenetic tree of the GPx1 family. |
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Keywords: | |
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