鳜鱼病毒传播途径的初步研究

采用PCR方法,追踪养殖鳜的亲鱼、子代、饵料鱼及与鳜养殖环境相关的水体生物和非生物因子中鳜鱼病毒的存在状况,并探讨鳜鱼病毒的传播途径。本研究中,鳜鱼塘水体中采集的除鳜以外的其他生物、底泥及水样的核酸抽提样本PCR扩增病毒结果呈阴性;感染病毒的亲鱼,其性腺中可检测出病毒,其子代组织病毒检测亦呈阳性。研究获得病毒可以在鳜体内呈潜伏状态存在和病毒可以垂直传播的一些证据。

Preliminary. study on the transmission route of Spercara chuatsi virus

In order to discuss the transmission of Spercara chuatsi virus (SCV), the existence of SCV was checked in the tissues of parent fish, their offspring, feedstuff fish, and in some factors relating to rearing environment of mandarin fish Spercara chuatsi by means of polymerase chain reaction (PCR). The SCV positive results were demonstrated in the parents' gonad and their offspring, while the SCV negative results were shown in all other samples collected in this experiment, including the diseased pond' s environmental factors such as plankton, upper-layer silt, and some aquatic insects. Some evidences were acquired in this investigation that SCV can exist in Spercara chuatsi in a latent state and can transmit from parent to its offspring.