| 烟草抗TMV基因连锁分子标记的筛选及在抗病资源筛选中的应用 |
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| 引用本文： | 高玉龙,肖炳光,童治军,许石剑. 烟草抗TMV基因连锁分子标记的筛选及在抗病资源筛选中的应用[J]. 分子植物育种, 2011, 0(5): 585-591 |
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| 作者姓名： | 高玉龙 肖炳光 童治军 许石剑 |
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| 作者单位： | 云南省烟草农业科学研究院;浙江大学;湖南农业大学 |
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| 基金项目： | 国家烟草专卖局项目(110201101010(JY-04));云南省烟草专卖局项目(06A03;08A05;2010YN02)共同资助 |
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| 摘 要： | 以抗TMV品种Coker176和感TMV品种K326为亲本,经杂交、自交获得F2作图群体.通过SRAP、SSR、N基因特异引物等分子标记分析结合田间TMV抗性鉴定,将TMV抗性基因定位于LG1连锁群上.TMV抗性基因位点位于标记N2和XSRP1x26之间,遗传距离分别是4.3 cM和9.6 cM.利用N2标记对84份烤...
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| 关 键 词： | 烟草 TMV 抗性基因 分子标记 |
Identification of Molecular Markers Linked to TMV Resistance Gene in Tobacco and Its Application in Screening Resistance Varieties |
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| Gao Yulong,Xiao Bingguang, Tong Zhijun,Xu Shijian. Identification of Molecular Markers Linked to TMV Resistance Gene in Tobacco and Its Application in Screening Resistance Varieties[J]. Molecular Plant Breeding, 2011, 0(5): 585-591 |
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| Authors: | Gao Yulong Xiao Bingguang Tong Zhijun Xu Shijian |
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| Affiliation: | 1,3 1 Yunnan Academy of Tobacco Agricultural Science, Yuxi, 653100; 2 Zhijiang University, Hangzhou, 310027; 3 Hunan Agricultural University, Changsha, 410128 |
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| Abstract: | One F2 population were constructed by cross between tobacco variety Coker176 (resistance to TMV) and K326 (susceptible to TMV). The resistance gene was located on linkage group one by molecular markers methods, such as SRAP, SSR, specific primers for N gene, combined with field resistance identification. The resistance gene was mapped between N2 (the genetic distance was 4.3 cM) and XSRP1x26 (the genetic distance was 9.6 cM). The eighty-three tobacco lines were amplified by N2 primer, and the result showed that the matching rate between N2 maker and resistance to TMV of lines was 97.62%. So, N2 maker could be applied to screen effectively resistance of TMV control by gene N. The germ plasms screened might be used as parent material of tobacco breeding. |
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| Keywords: | Tobacco Tobacco mosaic virus Resistance gene Molecular marker |
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