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| 新城疫病毒F基因真核表达载体的构建及瞬时表达 | |
| 引用本文： | 王 青,张彦明,王选年.新城疫病毒F基因真核表达载体的构建及瞬时表达[J].西北农林科技大学学报(社会科学版),2007,35(10):6-10. |
| 作者姓名： | 王 青 张彦明 王选年 |
| 作者单位： | 1. 西北农林科技大学,动物科技学院,陕西,杨凌712100 2. 河南科技学院,动物科学学院,河南,新乡,453003;河南省农业科学院,河南省动物免疫学重点实验室,河南,郑州450002 3. 河南省农业科学院,河南省动物免疫学重点实验室,河南,郑州450002 4. 河南科技学院,动物科学学院,河南,新乡,453003 5. 河南农业大学,牧医工程学院,河南,郑州,450002 |
| 基金项目： | 国家高技术研究发展计划(863计划) |
| 摘 要： | 应用PCR扩增出新城疫病毒F基因,将其克隆入pGEM-T载体,测序。然后将NDVF基因与高效的真核表达载体pcDNA4/HisMax相连,构建NDVF基因重组真核表达质粒pc4F,采用Superfect转染试剂将pc4F瞬时转染COS-7细胞,应用细胞免疫组化法检测其在细胞中的表达情况。结果表明,F基因能够在COS-7细胞中成功表达。 |
| 关 键 词： | 新城疫病毒 F基因 真核表达 |
| 文章编号： | 1671-9387（2007）10-006-05 |
| 收稿时间： | 2006/10/20 0:00:00 |
| 修稿时间： | 2006-10-20 |
Construction and transient expression of Newcastle disease virus F gene Eukaryotic vector in COS-7 cell line |
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| WANG Qing,ZHANG Yan-ming,WANG Xuan-nian,ZHANG Gai-ping,HU Jian-he,Yang Su-zhen,ZHAO Dong,XING Guang-xu,XU Yan-zhao,ZHAO Guang-hui,DUAN Yan-hua.Construction and transient expression of Newcastle disease virus F gene Eukaryotic vector in COS-7 cell line[J].Journal of Northwest Sci-Tech Univ of Agr and,2007,35(10):6-10. | |
| Authors: | WANG Qing ZHANG Yan-ming WANG Xuan-nian ZHANG Gai-ping HU Jian-he Yang Su-zhen ZHAO Dong XING Guang-xu XU Yan-zhao ZHAO Guang-hui DUAN Yan-hua |
| Institution: | 1 College of Animal Sciences and Technology,Northwest A & F University,Yangling,Shaanxi 712100,China;2 College of Animal Sciences,He’nan Institute of Science and Technology,Xinxiang,He’nan 453003,China;3 He’nan Key Laboratory for Animal Immunology,He’nan Academy of Agricultural Sciences,Zhengzhou,He’nan 450002,China;4 Technologic College of Animal Husbandry and Veterinary Science,Henan Agriculture University,Zhengzhou,He’nan 450002,China) |
| Abstract: | The F gene of NDV was amplified by PCR and cloned into pGEM-T Easy vector and sequenced. NDV F gene was, then,subcloned into high efficient eukaryotic vector pcDNA4/HisMax. The recombinant plasmid pc4F was transfected into COS-7 cells by using superfect transfection reagent. The NDV F protein expression in COS-7 cells was detected by immunocytochemistry. The transient expression of NDV-F protein provided the foundation for stable expression in a cell line,and for the future preparation of monoclonal antibodies that could be used to detect NDV and viral antigen variation. Results showed that F gene could be successflly experessed in COS-7 cells. |
| Keywords: | NDV F gene eukaryotic expression |
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