| 菌物DNA条形码技术的研究进展 |
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| 引用本文： | 刘淑艳,张 傲,李 玉.菌物DNA条形码技术的研究进展[J].华中农业大学学报,2012,31(1):121-126. |
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| 作者姓名： | 刘淑艳 张 傲 李 玉 |
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| 作者单位： | 吉林农业大学食药用菌教育部工程研究中心,长春,130118 |
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| 基金项目： | 国家自然科学基金项目（30970017和30300003）和教育部博士学科点专项科研基金项目（20102223120003） |
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| 摘 要： | DNA条形码技术是通过对一个DNA片段的分析比对,进而对物种进行鉴定的方法。研究的基因片段可以是1个或多个,进而完成物种识别和鉴定或者发现新物种。该项技术的优势在于整个鉴定过程不受研究对象不同发育阶段的影响。由于菌物具有独特而复杂的生活史,因此对菌物的鉴定工作相对较困难。世界上许多科学家都尝试过寻找适合于大多数菌物的标准DNA条码,但还没有找到能满足条件的基因片段。笔者对DNA条形码技术的发展历史、目的基因片段的筛选和研究进展等方面进行了综述,并讨论了DNA条形码技术存在的问题和今后研究的方向。
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| 关 键 词： | 菌物 DNA条形码 技术 分子鉴定 |
| 收稿时间： | 2011/6/24 0:00:00 |
Advances in research on the fungal DNA barcoding technology |
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| LIU Shu-yan,ZHANG Ao,LI Yu.Advances in research on the fungal DNA barcoding technology[J].Journal of Huazhong Agricultural University,2012,31(1):121-126. |
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| Authors: | LIU Shu-yan ZHANG Ao LI Yu |
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| Institution: | Engineering Research Center of Chinese Ministry of Education for Edible and Medicinal Fungi,Jilin Agricultural University,Changchun 130118,China |
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| Abstract: | DNA barcode is a technology which utilizes a short standardized DNA fragment to provide rapid DNA-based identification of unknown life samples. Its advantage is that the entire identification process would not be troubled by different life stages of the specimen, especially in fungi. The fungal identification is usually difficult because of their complex life cycle. In literature more studies have been conducted to screen a powerful suitable gene which could be used as the standard DNA barcode for fungi. The current review introduces the history of DNA barcode, DNA fragment screening, and the significance of DNA barcode to fungal taxonomy in China. |
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| Keywords: | fungi DNA barcode technology molecular identification |
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