基于ISSR标记的不同体色瓯江彩鲤种质鉴定

应用ISSR分子标记技术对5种体色瓯江彩鲤(Cyprinus carpio var.color)("全红"、"粉玉"、"粉花"、"麻花"和"大花")进行遗传多样性分析,筛选出的14条引物在5种体色瓯江彩鲤中共检测到125个位点,多态位点比率达68%,有效等位基因数为1.479 3,期望杂合度为0.273 9,Shannon多样性指数为0.401 0;5种不同体色瓯江彩鲤间的平均遗传距离为0.342 4,NJ法和UPMGA法对它们进行聚类分析显示"粉玉"、"粉花"和"全红"为一支,"麻花"和"大花"为另一支。应用引物848扩增的3个多态性位点构建了这5种不同体色瓯江彩鲤的DNA指纹图谱,可为不同体色瓯江彩鲤种质鉴定提供快捷、准确的鉴定结果。

Application of ISSR marker in germplasm identification of color patterns of Oujiang color carp

In this paper,the inter-simple sequence repeat(ISSR) technique were applied to analyze the genetic diversity among 5 color patterns of Oujiang color carp,designated as whole red,whole white,red color with small black spots,white color with large black patches,red color with large black patches,fourteen specific and stable primers were screened from 60 primers and a total of 125 loci were generated,of which 85 loci(68%) were polymorphic.The results indicated that effective number of alleles(Ne),expected heterozygosity(h) and Shannon’s information index(I) were 1.479 3,0.273 9 and 0.401 0,respectively.Genetic similarity(GS) and genetic distances(GD) were calculated by using Nei’s matching coefficient,the average GD among 5 color patterns of Oujiang color common carp is 0.342 4.When NJ and UPGMA cluster analysis were applied,Oujiang color carp were divided into two groups,named whole white,white color with large black patches and whole red in one group,red color with small black spots and red color with large black patches in the other group.Subsequently,the 3 polymorphic bands amplified from the primer 848 were employed to construct the DNA fingerprints of the 5 color patterns of Oujiang color carp.Among the constructed DNA fingerprints,each color pattern of Oujiang color carp had its unique fingerprinting pattern and could be easily distinguished from each other.Taken together,the results in this study show that the ISSR marker is a useful technique for identification of Oujiang color carp,offering the more correct and quicker results.