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Increased supply of boron to swiss chard plants did not affect the level of boron in a cell wall-bound form: A possible application to the diagnosis of nutrition of plants
Authors:Toru Matoh  Akira Matsuda  Ryo Akaike  Yoshitaka Hara  Masaru Kobayashi
Affiliation:1. Laboratory of Plant Nutrition, Division of Applied Life Sciences, Graduate School of Agriculture , Kyoto University , Kyoto , 606-8502 , Japan;2. Fertilizers Technical Department , Asahi Chemical Industry Co., Ltd. , Fuji , 416-8501 , Japan;3. Laboratory of Plant Nutrition, Division of Applied Life Sciences, Graduate School of Agriculture , Kyoto University , Kyoto , 606-8502 , Japan;4. Kyushu Agricultural Experimental Station , Chikugo , 833-0041 , Japan
Abstract:Swiss chard plants were water-cultured under various B levels ranging from 0.01 to 20 g B m-3 culture solutions for 4 weeks. The B content of the shoot increased with the increase in the B supply. The increase, however, was entirely ascribed to acetate buffer (20 mol m-3 pH 4.0)-soluble B, and the content of acetate buffer-insoluble, cell wall-bound B did not increase.. More than ca. 80% of the cell wall-bound B was associated with rhamnogalacturonan II under various B levels. The amount of acetate buffer-soluble B could be estimated from the B levels in the expressed leaf sap.
Keywords:Beta vulgaris  boron  cell wall  diagnosis  rhamnogalacturonan II
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