Isolation and partial characterization of endophytic diazotrophs associated with Japanese sugarcane cultivar |
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| Authors: | Constancio A Asis Jr Masatsugu Kubota Hiroyuki Ohta Yasuhiro Arima Vladimir K Chebotar Ken-ichi Tsuchiya |
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| Institution: | 1. United Graduate School , Tokyo University of Agriculture and Technology , Fuchu , 183-8509 , Japan;2. School of Agriculture , Ibaraki University , Ibaraki , 300-0393 , Japan;3. All Russia-Research Institute for Agricultural Microbiology , Shosse Podbelskogo 3, St. Petersburg-Pushkin 8, 189620 , Russia;4. National Institute of Agrobiological Resources , Tsukuba , 305-8602 , Japan |
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| Abstract: | Endophytic bacteria were isolated from the juice of a 6 month-old sugarcane cv. NiF-8, which was collected from Miyako, Okinawa and planted in a field lysimeter in Tsukuba, Ibaraki, Japan. The most probable number of N2-fixing endophytes was 4.5 × 105 cells per gram of fresh weight sample and 21 isolates have a positive reaction for ARA in an N2-free semi-solid medium with 10% crystallized sugarcane sugar and 0.5% sugarcane juice. Analyses of some of the biochemical properties of the N2-fixing isolates indicated that 13 isolates were putative strains of Acetobacter diazotrophicus, 4 isolates showed similar characteristics to those of Herbaspirillum seropedicae, and 4 isolates consisted of Herbaspirillum rubribalbicans-like strains. This study confirmed the existence of N2-fixing endophytic bacteria in the Japanese sugarcane cv. NiF-8. |
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| Keywords: | Acetobacter diazotrophicus biological nitrogen fixation Herbaspirillum species nitrogen-fixing endophytic diazotrophs sugarcane juice |
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