Changes in the microbial community structure in soils treated with a mixture of glucose and peptone with reference to the respiratory quinone profile |
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Authors: | Arata Katayama Hong-Ying Hu Mamie Nozawa Sayuri Takahashi Koichi Fujie |
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Affiliation: | 1. Research Center for Advanced Waste and Emission Management , Nagoya University , Nagoya, 464-8603, Japan;2. Department of Ecological Engineering , Toyohashi University of Technology , Toyohashi, 441-8580, Japan |
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Abstract: | Based on the respiratory quinone profile, changes in the structure of microbial communities in the soil samples from Nagoya University Farm were monitored after the treatment with 1% of a mixture of glucose and peptone. Samples of two soils differing in the fertilization history were examined: CF-soil with the application of only chemical fertilizers and FYM-soil with the application of only farmyard manure at a high rate. In the CF-soil, the amount of water-soluble organic carbon (WOC), indicator of the mixture of glucose and peptone, decreased to the original level after 14 d. After 7 d, the soil pH reached the maximum level, then decreased gradually. Changes in the inorganic nitrogen levels in the water extract also reflected the 14-d period of mineralization. The amount of respiratory quinones reached maximum levels after 7 d and gradually decreased, reflecting the changes in the microbial biomass. The quinone composition significantly changed during the 14-d period and returned to a profile similar to the original one after 28 d. Diversity of quinones significantly decreased during the 14-d period due to the predominance of ubiquinone with 9 isoprenoid units. In the FYM-soil, the amount of WOC decreased to the original level after 1 d, and the pH and inorganic nitrogen levels in the water extract reflected the one-day mineralization period, and nitrification started after 3 d. Although the amount of quinones indicated an increase in the microbial biomass for 14 d, the quinone composition did not change. These findings suggested that long-term application of farmyard manure resulted in stable microbial communities in response to the incorporation of organic matter in soil. |
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Keywords: | respiratory quinones soil pH temporal changes of microbiota water-soluble organic carbon |
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