Negative correlation between the ratio of K+ to Na+ and proline accumulation in tobacco suspension cells |
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Authors: | Eiji Okuma Kenji Soeda Miho Fukuda Mikiro Tada Yoshiyuki Murata |
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Affiliation: | 1. Graduate School of Natural Science and Technology , Okayama University , Okayama, 700-8530, Japan;2. Department of Agriculture , Okayama University , Okayama, 700-8530, Japan |
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Abstract: | Abstract The concentrations of K+, Na+, and proline and the ratio of K+ to Na+ (K+ / Na+) were analyzed in NaCl-unadapted and NaCl-adapted tobacco (Nicotiana tabacum) cells in suspension culture. At 3 to 5 d after inoculation, the NaCl-unadapted cells cultured in 100 mmol L?1 NaCl saline culture medium (Na100 medium) accumulated 28.7 mmol L?1 proline with a low ratio of K+ to Na+ (= 2.8) and the NaCl-adapted cells cultured in the Na100 medium contained 6.28 mmol L?1 proline with a high K+ / Na+ ratio (≧ 7.5). The contents of amino acids for the NaCl-adapted cells in the Na100 medium were similar to those for the NaCl-unadapted cells in a modified LS medium (standard medium). At 14 d after inoculation, the NaCl-unadapted cells in the Na100 medium contained 4.77 mmol L?1 proline and restored the K+ / Na+ ratio from 2.8 to 6.2. These results indicate the presence of a negative correlation between the K+ / Na+ ratio and proline accumulation and suggest that a balance between the K+ / Na+ ratio and proline accumulation may be the factor involved in determining the salt tolerance of plant cells. |
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Keywords: | K+ / Na+ ratio Nicotiana tabacum proline salt stress tobacco |
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