Optimization of Medium Composition for Production of Recombinant Calf Chymosin from Kluyveromyces lactis in Submerged Fermentation |
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| Authors: | FENG Zhen ZHANG Hongwei ZHANG Yanhe HU Sen JIANG Qian College of Food Science Northeast Agricultural University Harbin China Harbin Veterinary Research Institute Chinese Academy of Agricultural Sciences Harbin China |
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| Affiliation: | FENG Zhen1,ZHANG Hongwei1,ZHANG Yanhe2,HU Sen2,and JIANG Qian2 1 College of Food Science,Northeast Agricultural University,Harbin 150030,China 2 Harbin Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Harbin 150001,China |
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| Abstract: | Response surface methodology was applied to optimize medium components for production of recombinant calf chymosin by Kluyveromyces lactis GG799.The previous data indicated that the most suitable carbon source,nitrogen source,salt and vitamin were glucose,yeast extract,KH2PO4 and Ca D-Pantothenate,respectively.The concentration of four media components were optimized by using central composite design of response surface methodology.The optimum medium composition for recombinant calf chymosin production was ... |
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| Keywords: | chymosin Kluyveromyces lactis response surface methodology medium optimization |
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