Evaluation of selective media for primary isolation of Treponema hyodysenteriae and Treponema innocens |
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| Authors: | Z M Szynkiewicz M Binek |
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| Affiliation: | 1. School of Life Sciences, North Maharashtra University, Jalgaon, 425001, MS, India;2. G. K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Pushchino, Moscow Region, 142290, Russia;1. Department of Orthopedics, The First Affiliated Hospital of Chengdu Medical College, Chengdu Medical College, No. 278 Middle of Baoguang Road, Xindu District, Chengdu, Sichuan, 610500, China;2. Department of Orthopedics, West China Hospital, Sichuan University, 37# Wainan Guoxue Road, Chengdu, Sichuan, 610041, China;3. Department of Endocrinology, The First Affiliated Hospital of Chengdu Medical College, Chengdu Medical College, No. 278 Middle of Baoguang Road, Xindu District, Chengdu, Sichuan, 610500, China;1. Department of Animal Breeding and Genetics Science, Swedish University of Agricultural Science, SLU-Global Bioinformatics Centre, Uppsala, SE 750 07, Sweden;2. Department of Bioinformatics and Biotechnology, International Islamic University, Islamabad, Pakistan;3. Department of Microbiology, National Veterinary Institute, SE-751 89 Uppsala, Sweden;4. Department of Animal Health and Antimicrobial Strategies, National Veterinary Institute, SE-751 89 Uppsala, Sweden;5. Department of Biomedical Sciences and Veterinary Public Health, Swedish University of Agricultural Science, SE-750 07 Uppsala, Sweden;1. Department of Basic Research, Indian Spinal Injuries Centre, New Delhi, India;2. Medical Director and Chief of Spine Unit, Indian Spinal Injuries Centre, New Delhi, India |
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| Abstract: | A total of 2450 samples of feces, intestinal contents and colon mucosal scrapings were bacteriologically examined. A total of 53 strains of Treponema sp. were isolated, and 45 strains of Bacteroides sp., 30 strains of E. coli, 30 strains of Micrococcus sp. and 10 strains of Streptococcus D isolates were randomly selected. Growth promoting studies showed statistically significant stimulation of Treponema sp. growth by yeast extract, chicken egg yolk and rumen fluid. Different growth inhibitors were also tested. For selective medium the following inhibitors were selected: spectinomycin, colistin, vancomycin, brilliant green. Optimal concentrations of these inhibitors in the medium were determined. Finally TSA medium supplemented with 0.05% yeast extract, 5% bovine blood, 0.01% DTT, 400 micrograms spectinomycin, and 250 micrograms/ml vancomycin, appeared to be optimal selective medium for intestinal Treponema sp. isolation. Quantitative studies showed that the number of Treponema C.F.U. on Songers et al. medium with spectinomycin and on spectinomycin-vancomycin medium, did not differ significantly. The number of overgrowing bacteria was statistically significantly lower on spectinomycin-vancomycin medium, than Songers et al. selective medium with spectinomycin. The TSA supplemented with blood, yeast extract 50 micrograms/ml of colistin and 1 microgram/ml of brilliant green was less selective than spectinomycin-vancomycin medium and inhibited some strains of Treponema sp. In the case of spectinomycin-vancomycin resistant of overgrowing bacteria, colistin-brilliant green medium may be suitable for isolation of Treponema sp. |
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