| 白背毛木耳杂交育种及杂交子选育 |
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| 引用本文： | 冀宏,刘萍,朱清,姚璐晔,徐兵. 白背毛木耳杂交育种及杂交子选育[J]. 中国食用菌, 2010, 29(6): 18-21 |
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| 作者姓名： | 冀宏 刘萍 朱清 姚璐晔 徐兵 |
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| 作者单位： | 常熟理工学院生物与食品工程学院,江苏常熟215500 |
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| 基金项目： | 江苏省科技厅苏北科技专项资助项目，苏州市科技发展计划项目 |
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| 摘 要： | 为了获得性状优良的毛木耳新品种,进行了杂交育种。实验以钩悬法收集毛木耳"丰2"子实体单孢子并培养获得其单核菌丝。采用单孢杂交(两点法)进行杂交培养,镜检"锁状联合"初选杂交子;通过与亲本的拮抗实验和酯酶同工酶电泳酶谱比对分析确认杂交子,并经出耳试验验证其结实性。实验结果显示,种内杂交的不同单核菌丝较易亲和并产生具结实能力的杂交子。
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| 关 键 词： | 白背毛木耳 单核菌丝 杂交子 酯酶同工酶电泳 |
Cross Breeding of Auricularia polytricha and Identification of the Hybrid Strain |
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| JI Hong,LIU Ping,ZHU Qing,YAO Lu-ye,XU Bing. Cross Breeding of Auricularia polytricha and Identification of the Hybrid Strain[J]. Edible Fungi of China, 2010, 29(6): 18-21 |
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| Authors: | JI Hong LIU Ping ZHU Qing YAO Lu-ye XU Bing |
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| Affiliation: | (College of Biology ＆ Food Engineering,ChangShu Institute of Technology,Changshu Jiangsu 215500) |
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| Abstract: | The monospores of Auricularia polytricha were collected.2 monokaryon strains were mated by random.At first,the hybrids with clamp connection were selected by microscopy.Then hybrids were confirmed by antagonism experiments and esterase isozyme analysis.In the end,hybrids were cultivated.It’s indicated that hybrids could be obtained easily from intraspecific cross,which also had good fruiting ability. |
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| Keywords: | Auricularia polytricha Monokaryon-mycelia hybrid Esterase-isoenzyme electrophoresis |
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