马铃薯中薯5号和鄂马铃薯一号转苗节数及苗龄对试管种薯形成的影响

为获得马铃薯推广品种"中薯5号"(中5)和"鄂马铃薯一号"(E1)微型种薯高效生产的重要参数,用脱毒试管苗为材料,设置每盒20节、30节、40节和50节4种转节苗数处理及4 w和5 w两个不同基础苗苗龄处理,研究了不同转苗节数条件及不同基础苗苗龄下生长材料的单盒薯数、大粒和小粒薯数及平均薯重等指标。结果表明,每盒植入40个腋芽数及基础苗苗龄为4 w是中5和E1两个马铃薯品种之微型种薯工厂化生产高效且低成本的适宜参数。此外,通过培养后期污染和未污染的对比研究证明,防止污染是规模化生产中操作和管理的重点。

Study on Virus-free Seed Microtuber Production with Different Number of Nodal Segment and Age of Plantlet in Culturing of Potato Varieties Zhong 5 and E1

In order to obtain key parameters for effective production of microtuber of "Zhong shu 5 hao"(zhong 5) and "E-malingshu 1 hao"(E1),two varieties of potato widely popularized in faming,referential data such as microtubules number per box,numbers of large and small tuber,average weight of tuber etc.under different seedling conditions and plant ages were studied using the virus-free plantlets of the varieties by 4 treatments with 20,30,40 and 50 single nodal segments per culturing box and by 2 treatments with culture durations of 4w and 5w for the plantlets separately.The results showed that 40 single-node segments per box and the 4w of basis plants are appropriate parameters to achieve higher productivity with low cost in factory production of microtuber for "zhong 5"and "E1".In addition,the comparative study on cultivation of the late pollution and non-pollution was conducted and the result suggested that preventing pollution is an important issue,so more attention should be paid in operation and management in the course of large-scale production of microtuber.