蝉拟青霉分生孢子萌发的影响因子

研究了不同营养、温度、湿度、光照、pH值等条件对蝉拟青霉LB菌株分生孢子萌发的影响.结果表明:2%葡萄糖+1%蛋白胨溶液制成孢子悬浮液的萌发率最高达95.09%;25～27℃是蝉拟青霉LB菌株分生孢子萌发的适宜温度条件;分生孢子萌发所需湿度为90%～100%,当湿度低于90%时萌发受到抑制;分生孢子在pH 6～7的中性至弱酸环境下萌发最好;光照对孢子萌发无影响;紫外线对孢子有明显的杀伤作用,随紫外线照射时间的延长,孢子萌发率明显降低,经紫外线照射后孢子有萌发迟缓的现象.

Factors Affected Conidia Germination of Paecilomyces cicadae

Effects of different nutrition, temperature, moisture, light, and pH were studied on conidia germination of Paecilomyces cicadae LB. The results showed that the nutrition (2% glucose + 1% peptone)strongly promoted conidia germination, and reached the highest germination rate 95.09%. The optimum temperature for the germination of the spore was 25～27℃.The relative humidity for germination was between 90%～100%, while less than 90% spore could not germinate. The suitable pH was 6～7 for spore germination. There was no effect from light for spore germination. UV obviously had killing effects on spore, the germination rate of spore decreased evidently along with the longer exposure time, spore had retarded germination when treated by UV, with germination rate 78.10% in 24 hours treated 20 min, 52.73% for 40 min, and 23.36% for 60 min.