金银花花粉生活力测定及贮藏研究

[目的]对不同贮藏条件下金银花花粉生活力进行测定,以期为金银花生产、科研提供参考。[方法]以金银花新鲜花粉为试材,通过I-KI染色法、TTC（氯化三苯基四氮唑）染色法、培养基萌发试验法对花粉生活力进行测定,并研究了不同贮藏条件对花粉萌发的影响。[结果]培养基中添加不同浓度的蔗糖和硼酸对花粉的萌发有促进作用,其浓度分别为150、30mg/L时花粉的萌发率最大值为75.23%和52.78%;I-KI染色法不宜用于金银花花粉生活力测定;采用TTC染色法测定金银花花粉生活力达80.46%。[结论]TTC染色法测定金银花花粉生活力相对准确且简便易行。在金银花花粉贮藏过程中低温贮藏（-80℃）好于冷藏（4℃、-23℃）,不宜常温贮藏。

Study on Pollen Viability Determination and Storage of Honeysuckle

[Objective] Pollen viability of honeysuckle was determined at different storage conditions,which provided a reference for producing and researching of honeysuckle.[Method] Taken the fresh pollen of honeysuckle as an experimental material,I-KI staining,TTC staining and culture medium germination methods were used to determine its fresh pollen ability.Effects of different storage on the pollen germination were studied.[Result] The results showed that addition different concentration of sucrose and boric acid to culture media had promoted effects on the pollen germination.When the concentrations were 150 and 30 mg/L,the germination rate reached the maximum,which were 75.23% and 52.78%.I-KI staining was not suitable for the determination of pollen viability of honeysuckle,while TTC staining was suitable for it and rates reached 80.46%.[Conclusion] This method is relative correct and simple.It was better mid-low temperature storage(-80 ℃)than cold storage(4 ℃、-23 ℃),it was not suitable for the normal condition.