| 气相色谱-质谱法测定发酵乳中氨基甲酸乙酯含量 |
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| 引用本文： | 陈同强,梁锋,吴海智,向俊,宋阳,荆辉华,袁列江,李灿,李凯龙. 气相色谱-质谱法测定发酵乳中氨基甲酸乙酯含量[J]. 乳业科学与技术, 2022, 45(6): 18-21. DOI: 10.7506/rykxyjs1671-5187-20220510-029 |
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| 作者姓名： | 陈同强 梁锋 吴海智 向俊 宋阳 荆辉华 袁列江 李灿 李凯龙 |
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| 作者单位： | （1.湖南省产商品质量检验研究院, 湖南 长沙 410007；2.中南林业科技大学食品科学与工程学院, 湖南 长沙 410004；3.中南大学化学化工学院, 湖南 长沙 410083；4.湖南省农业科学院植物保护研究所, 湖南 长沙 410125） |
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| 基金项目： | 湖南省自然科学基金项目（2022JJ90024）；湖南省市场监督管理局科技计划项目（2022KJJH41） |
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| 摘 要： | 采用气相色谱-质谱法定量，建立测定发酵乳中氨基甲酸乙酯含量的分析方法。通过优化前处理条件，同时考察方法的线性关系、精密度、加标回收率、检出限及定量限等指标，内标法定量。结果表明：该方法在0～200?ng/mL范围内线性良好（r＞0.999），氨基甲酸乙酯检出限为2.0?μg/kg，定量限为5.0?μg/kg，加标回收率为89.9%～105.2%，相对标准偏差小于10%。该方法灵敏、准确、重复性好，能满足发酵乳中氨基甲酸乙酯含量的测定要求。
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| 关 键 词： | 气相色谱-质谱；发酵乳；氨基甲酸乙酯；含量测定 |
Determination of Ethyl Carbamate in Fermented Milk by Gas Chromatography-Mass Spectrometry |
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| CHEN Tongqiang,LIANG Feng,WU Haizhi,XIANG Jun,SONG Yang,JING Huihua,YUAN Liejiang,LI Can,LI Kailong. Determination of Ethyl Carbamate in Fermented Milk by Gas Chromatography-Mass Spectrometry[J]. JOURNAL OF DAIRY SCIENCE AND TECHNOLOGY, 2022, 45(6): 18-21. DOI: 10.7506/rykxyjs1671-5187-20220510-029 |
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| Authors: | CHEN Tongqiang LIANG Feng WU Haizhi XIANG Jun SONG Yang JING Huihua YUAN Liejiang LI Can LI Kailong |
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| Affiliation: | (1.Hunan Province Produce Commodity Quality Testing and Research Institute, Changsha 410007, China; 2.College of Food Science and Engineering, Central South University of Forestry Science and Technology, Changsha 410004, China; 3.College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, China; 4.Hunan Plant Protection Institute, Hunan Academy of Agricultural Science, Changsha 410125, China) |
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| Abstract: | An analytical method for the determination of ethyl carbamate in fermented milk was established by gas chromatography-mass spectrometry (GC-MS). Sample pretreatment conditions were optimized. The analytical figures of merit of the proposed method including linear relationship, precision, spiked recovery, detection limit and quantitation limit were evaluated. Ethyl carbamate was quantitated using an external standard. The linearity of this method was good in the range of 0–200 ng/mL (r > 0.999), and the detection limit and quantitation limit of ethyl carbamate were 2.0 and 5.0 μg/kg, respectively. The recovery of the added ethyl carbamate ranged from 89.9% to 105.2%, with relative standard deviation (RSD) less than 10%. This method is sensitive, accurate and repeatable, and can meet the requirements for the determination of ethyl carbamate in fermented milk. |
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| Keywords: | gas chromatography-mass spectrometry fermented milk ethyl carbamate quantitation, |
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