| 植物乳杆菌胞外多糖的单糖组成及抗氧化活性 |
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| 引用本文： | 朱玉婷,文欣,向俊,郭锦材,荆辉华,李灿,陈同强. 植物乳杆菌胞外多糖的单糖组成及抗氧化活性[J]. 乳业科学与技术, 2022, 45(6): 7-11. DOI: 10.7506/rykxyjs1671-5187-20220510-030 |
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| 作者姓名： | 朱玉婷 文欣 向俊 郭锦材 荆辉华 李灿 陈同强 |
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| 作者单位： | （1.三二○一医院药学部, 陕西 汉中 723000；2.湖南省产商品质量检验研究院, 湖南 长沙 410007；3.中南大学化学化工学院, 湖南 长沙 410083；4.长沙市口腔医院, 湖南 长沙 410006；5.中南林业科技大学食品科学与工程学院, 湖南 长沙 410004） |
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| 基金项目： | 湖南省市场监督管理局科技计划项目（2021KJJH06）；湖南省自然科学基金科卫联合项目（2021JJ70062）；长沙市自然科学基金项目（kq2014019）；湖南省保健专项资金科研课题项目（B2020-07）；湖南省医学会临床药学科研基金项目（B202012） |
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| 摘 要： | 分析植物乳杆菌胞外多糖的单糖组成，同时对其抗氧化活性进行研究。以植物乳杆菌发酵液为原料，通过水提醇沉方法得到植物乳杆菌粗多糖（DZ），经DEAE-52纤维素柱分离纯化收集得到DZ-1与DZ-2 2 种多糖组分，采用气相色谱法对DZ-1与DZ-2中单糖组成进行分析，测定其1,1-二苯基-2-三硝基苯肼（1,1-diphenyl-2-picrylhydrazyl，DPPH）自由基、羟自由基和2,2’-联氮-双-(3-乙基苯并噻唑啉-6-磺酸)（2,2’-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid)，ABTS）阳离子自由基清除率，对2 种多糖组分的抗氧化活性进行研究。结果表明：DZ-1为仅含葡萄糖的均多糖，DZ-2则为由阿拉伯糖与半乳糖组成的杂多糖，其单糖组成及百分比含量比值为阿拉伯糖∶半乳糖=46.03∶53.89；抗氧化实验结果表明，DZ-1与DZ-2均具有一定的DPPH自由基、羟自由基和ABTS阳离子自由基清除能力。
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| 关 键 词： | 植物乳杆菌；胞外多糖；分离纯化；单糖组成；抗氧化 |
Monosaccharide Composition and Antioxidant Activity of Exopolysaccharides of Lactobacillus plantarum |
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| ZHU Yuting,WEN Xin,XIANG Jun,GUO Jincai,JIN Huihua,LI Can,CHEN Tongqiang. Monosaccharide Composition and Antioxidant Activity of Exopolysaccharides of Lactobacillus plantarum[J]. JOURNAL OF DAIRY SCIENCE AND TECHNOLOGY, 2022, 45(6): 7-11. DOI: 10.7506/rykxyjs1671-5187-20220510-030 |
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| Authors: | ZHU Yuting WEN Xin XIANG Jun GUO Jincai JIN Huihua LI Can CHEN Tongqiang |
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| Affiliation: | (1.Department of Pharmacy, 3201 Hospital, Hanzhong 723000, China;2.Hunan Province Produce Commodity Quality Testing and Research Institute, Changsha 410007, China; 3.College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, China; 4.Changsha Stomatological Hospital, Changsha 410006, China; 5.College of Food Science and Engineering, Central South University of Forestry Science and Technology, Changsha 410004, China) |
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| Abstract: | The monosaccharide composition and antioxidant activity of extracellular polysaccharides from Lactobacillus plantarum were analyzed. Crude polysaccharides were obtained from the fermentation broth of Lactobacillus plantarum by water extraction and alcohol precipitation and were fractionated into two fractions (DZ-1 and DZ-2) by DEAE-52 cellulose column chromatography. The monosaccharide components of DZ-1 and DZ-2 were analyzed by gas chromatography, and their antioxidant activity was tested using 1,1-diphenyl-2-picrylhy drazyl (DPPH), hydroxyl radical, and 2,2’-diazo bis-3-ethyl benzothiazoline-6-sulfonic acid (ABTS) cation radical scavenging assays. DZ-1 was a homopolysaccharide composed of only glucose units, while DZ-2 was a heteropolysaccharide composed of arabinose and galactose with a content ratio of 46.03:53.89. Both DZ-1 and DZ-2 had the ability to scavenge DPPH, hydroxyl and ABTS cation radicals. |
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| Keywords: | Lactobacillus plantarum exopolysaccharide separation and purification monosaccharide composition antioxidant activity, |
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