应用RP-HPLC法测定野山杏果肉黄酮类化合物

[目的]分析测定新疆野山杏果肉中4种黄酮类化合物(原花青素、芦丁、柚皮苷二氢查尔酮、槲皮素).[方法]运用高效液相色谱分析方法,采用Hubble C18(4.6 mm×250 mm,5μm)色谱柱,流动相A(甲醇):B(0.5％磷酸水溶液)=45:55,在1.0 mL/min流速下等度洗脱,分离新疆野山杏果肉中4种黄酮...

Determination of Flavonoids in Wild Apricot Flesh by RP-HPLC

【Objective】 To establish a method for simultaneous determination of four flavonoids (proanthocyanidins, rutin, naringin dihydrochalcone and quercetin) in the sarcocarp of Xinjiang wild apricot by high performance liquid chromatography.【Method】 Four flavonoids from Xinjiang wild apricot sarcocarp were separated by Hubble C18 (4.6 mm ×250 mm, 5 m) column, mobile phase A (methanol)∶ B (0.5% phosphoric acid solution) = 45∶55, elution at a flow rate of 1.0 mL/min. The method was validated by instrument precision, stability, repeatability and recovery.【Result】 The chromatographic peaks of proanthocyanidin, rutin and quercetin were well separated within 20 minutes. The linear range was 0.52-56 g/mL (r = 0.999,8-1). The recovery was 92.13%-96.70% and RSD was 0.89%-1.96%. The content of proanthocyanidins, rutin and quercetin were 7.325 mg/kg, 0.630 mg/kg and 0.009,58 mg/kg respectively. Naringin dihydrochalcone was not detected under this condition.【Conclusion】 The method is simple, rapid, accurate, stable and reproducible. It is suitable for the separation and determination of three flavonoids in Xinjiang wild apricot flesh.