| 玉米BADH基因的克隆与序列分析 |
| |
| 引用本文： | 余爱丽,徐景升,周会,张木清,陈如凯. 玉米BADH基因的克隆与序列分析[J]. 分子植物育种, 2004, 2(3): 365-368 |
| |
| 作者姓名： | 余爱丽 徐景升 周会 张木清 陈如凯 |
| |
| 作者单位： | 福建农林大学农业部甘蔗生理生态与遗传改良重点开放实验室,福州,350002;福建农林大学农业部甘蔗生理生态与遗传改良重点开放实验室,福州,350002;福建农林大学农业部甘蔗生理生态与遗传改良重点开放实验室,福州,350002;福建农林大学农业部甘蔗生理生态与遗传改良重点开放实验室,福州,350002;福建农林大学农业部甘蔗生理生态与遗传改良重点开放实验室,福州,350002 |
| |
| 摘 要： | Betaine was accumulated as a nontoxic and protective osmolyte in water-dificit and salt-stressed plants. Betaine aldehyde dehydrogenase for glycine betaine synthesis is an important enzyme. The BADH gene of Maize was cloned by RT-PCR and RACE. The gene is 1 762 bp in length, including an open reading frame of 1 515 bp.Its nucleotide sequence shares 95% with the the partial cDNA of BADHI from Sorghum bicolor. Its deduced amino acid sequence contains the conserved domain sequence “VTLELGGKSP“ of ALDH, and a tripeptide SKL at its C-terminal, a signal targetting to the microbodies. The phylogenetic tree of 20 BADHs was constructed,which corresponds to the classical botanical division of plant, the BADH of maize is closest to the one of Oryza Sativa.
|
| 关 键 词： | 玉米 BADH基因 基因克隆 序列分析 |
Cloning and Sequencing of BADH Gene from Maize(Zea mays) |
| |
| Yu Aili Xu Jingsheng Zhou Hui Zhang Muqing Chen RukaiKey Laboratory of Eco-physiology and Genetic Improvement for Sugancane,Ministry of Agriculture,Fujian Agricultural and Forestry University,Fuzhou. Cloning and Sequencing of BADH Gene from Maize(Zea mays)[J]. Molecular Plant Breeding, 2004, 2(3): 365-368 |
| |
| Authors: | Yu Aili Xu Jingsheng Zhou Hui Zhang Muqing Chen RukaiKey Laboratory of Eco-physiology Genetic Improvement for Sugancane Ministry of Agriculture Fujian Agricultural Forestry University Fuzhou |
| |
| Affiliation: | Yu Aili Xu Jingsheng Zhou Hui Zhang Muqing Chen RukaiKey Laboratory of Eco-physiology and Genetic Improvement for Sugancane,Ministry of Agriculture,Fujian Agricultural and Forestry University,Fuzhou,350002 |
| |
| Abstract: | Betaine was accumulated as a nontoxic and protective osmolyte in water-dificit and salt-stressed plants. Be-taine aldehyde dehydrogenase for glycine betaine synthesis is an important enzyme. The BADH gene of Maize was cloned by RT-PCR and RACE. The gene is 1 762 bp in length, including an open reading frame of 1 515 bp. Its nucleotide sequence shares 95% with the the partial cDNA of BADH1 from Sorghum bicolor. Its deduced amino acid sequence contains the conserved domain sequence "VTLELGGKSP" of ALDH, and a tripeptide SKL at its C-terminal, a signal targetting to the microbodies. The phylogenetic tree of 20 BADHs was constructed , which corresponds to the classical botanical division of plant, the BADH of maize is closest to the one of Oryza Sativa,GenBank accession No. AY587278. |
| |
| Keywords: | BADH Zea mays RACE |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
