| 高赖氨酸蛋白基因遗传转化水稻的研究 |
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| 引用本文： | 蒋家焕,刘峰,许明,黄志伟,谢丽雪,郑金贵.高赖氨酸蛋白基因遗传转化水稻的研究[J].福建农林大学学报(自然科学版),2006,35(6):615-618. |
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| 作者姓名： | 蒋家焕 刘峰 许明 黄志伟 谢丽雪 郑金贵 |
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| 作者单位： | 1. 福建农林大学农产品品质研究所,福建,福州,350002;福建省农科院水稻研究所,福建,福州,350018
2. 福建农林大学农产品品质研究所,福建,福州,350002 |
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| 基金项目： | 国家重点基础研究发展计划(973计划);国家自然科学基金;福建省科技厅重大专项前期研究项目;福建省科技厅科研项目 |
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| 摘 要： | 采用农杆菌介导的方法将高赖氨酸蛋白基因导入台粳9号幼胚诱导的胚性愈伤组织中,经潮霉素筛选,抗性愈伤分化成苗,共获得2株转基因植株.对这些植株及后代植株进行GUS染色和PCR及PCR Southern b lot检测分析,确定高赖氨酸蛋白基因已整合到台粳9号基因组中,并能稳定遗传表达.经检测,转基因水稻糙米赖氨酸含量为0.349%,比对照提高29.3%;转基因水稻秸秆赖氨酸含量为0.341%,比对照提高8.3%.
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| 关 键 词： | 台粳9号 高赖氨酸蛋白基因 遗传转化 农杆菌 |
| 文章编号： | 1671-5470(2006)06-0615-04 |
| 收稿时间： | 2006-08-21 |
| 修稿时间： | 2006-10-11 |
Research of transforming Lysine-rich protein gene to rice |
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| JIANG Jia-huan,LIU Feng,XU Ming,HUANG Zhi-wei,XIE Li-xue,ZHENG Jin-gui.Research of transforming Lysine-rich protein gene to rice[J].Journal of Fujian Agricultural and Forestry University,2006,35(6):615-618. |
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| Authors: | JIANG Jia-huan LIU Feng XU Ming HUANG Zhi-wei XIE Li-xue ZHENG Jin-gui |
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| Institution: | 1. Agriculture Product Quality Institute,Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China; 2. Rice Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350018, China |
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| Abstract: | Lysine-rich protein gene was transformed to the embryonic callus tissue of Taijing 9 with Agrobacterium-mediated method.After resistance screening by hygromix,the resistant calli differentiated to seedlings,and 2 transgenic plants were obtained.PCR testing and GUS staining analysis showed that Lysine-rich protein gene had been transformed to the genome of Taijing 9,and it could be stably expressed in its next generation.The test results showed that lysine content in unpolished grains of transgenic rice was(0.349%,) improved 29.3% compared to control;and lysine content in transgenic rice straw was 0.341%,enhanced 8.3% compared to control. |
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| Keywords: | Taijing 9 Lysine-rich protein gene genetic transformation Agrobacterium |
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