NO对氮胁迫棉花幼苗根系形态的调控效应

以‘农大棉8号’为试验材料,在水培缺氮的情况下,利用一氧化氮(Nitric oxide,NO)的供体硝普钠(Sodi-um nitroprusside,SNP)处理棉花幼苗,研究了外源NO对氮素胁迫条件下棉花幼苗根系形态的调控效应。结果表明:外源NO能够增加氮素胁迫下棉花根长、根表面积、根体积、主根直径和根干重。低浓度的NO(SNP浓度50～100μmol/L)能显著提高根表面积9.39%、根体积10.78%、主根直径8.63%、根系干重39.2%;高浓度NO对氮素胁迫缓解效应较弱。因此,在本试验条件下,低浓度的NO(SNP浓度50～100μmol/L)表现对氮素胁迫下棉花根系生长具有良好促进效应,可能利于增强棉花的抗逆性。

Regulatory effects of exogenous nitric oxide on root morphology in cotton seedlings under nitrogen stress

The effects of sodium nitroprusside(SNP,an exogenous nitric oxide donor) on root morphology in cotton seedlings under nitrogen stress were studied by using nutrient solution culture.Exogenous nitric oxide can promote cotton seedlings'root length,root surface area,root volume,taproot diameter and root dry weight.Lower concentration of NO can at least significantly increase the root surface area by 9.39%,root volume by 10.78%,taproot diameter by 8.63% and root dry weight by 39.2%.High concentration can reduce the effect.In this experimental conditions,lower concentration of NO(SNP:50-100μmol/L) has the best effect on mitigating cotton seedlings under nitrogen stress,which may enhance the resistance of cotton.