Soil microbial biomass response to woody plant invasion of grassland

Woody plant proliferation in grasslands and savannas has been documented worldwide in recent history. To better understand the consequences of this vegetation change for the C-cycle, we measured soil microbial biomass carbon (C_mic) in remnant grasslands (time 0) and woody plant stands ranging in age from 10 to 130 years in a subtropical ecosystem undergoing succession from grassland to woodlands dominated by N-fixing trees. We also determined the ratio of SMB-C to soil organic carbon (C_mic/C_org) as an indicator of soil organic matter quality or availability, and the metabolic quotient (qCO₂) as a measure of microbial efficiency. Soil organic carbon (C_org) and soil total nitrogen (STN) increased up to 200% in the 0–15 cm depth increment following woody plant invasion of grassland, but changed little at 15–30 cm. C_mic at 0–15 cm increased linearly with time following woody plant encroachment and ranged from 400 mg C kg^?1 soil in remnant grasslands up to 600–1000 mg C kg^?1 soil in older (>60 years) woody plant stands. C_mic at 15–30 cm also increased linearly with time, ranging from 100 mg C kg^?1 soil in remnant grasslands to 400–700 mg C kg^?1 soil in older wooded areas. These changes in C_mic in wooded areas were correlated with concurrent changes in stores of C and N in soils, roots, and litter. The C_mic/C_org ratio at 0–15 cm decreased with increasing woody plant stand age from 6% in grasslands to <4% in older woodlands suggesting that woody litter may be less suitable as a microbial substrate compared with grassland litter. In addition, higher qCO₂ values in woodlands (0.8 mg CO₂-C g^?1 C_mic h^?1) relative to remnant grasslands (0.4 mg CO₂-C g^?1 C_mic h^?1) indicated that more respiration was required per unit of C_mic in wooded areas than in grasslands. Observed increases in C_org and STN following woody plant encroachment in this ecosystem may be a function of both greater inputs of poor quality C that is relatively resistant to decay, and the decreased ability of soil microbes to decompose this organic matter. We suggest that increases in the size and activity of C_mic following woody plant encroachment may result in: (a) alterations in competitive interactions and successional processes due to changes in nutrient dynamics, (b) enhanced formation and maintenance of soil physical structures that promote C_org sequestration, and/or (c) increased trace gas fluxes that have the potential to influence atmospheric chemistry and the climate system at regional to global scales.