| 猪圆环病毒2型山东分离株全基因组的克隆与序列分析 |
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| 引用本文： | 柳美玲,秦晓冰,王爱华,牛晋国,单虎. 猪圆环病毒2型山东分离株全基因组的克隆与序列分析[J]. 动物医学进展, 2008, 29(1): 13-17 |
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| 作者姓名： | 柳美玲 秦晓冰 王爱华 牛晋国 单虎 |
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| 作者单位： | 青岛农业大学动物科技学院,山东青岛,266109;云南农业大学动物科技学院,云南昆明,650201;山西省农业科学院畜牧兽医研究所,山西太原,030032 |
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| 摘 要： | 根据猪圆环病毒2型(PCV-2)全基因组序列,设计一对特异性引物,从疑似断奶仔猪多系统衰竭综合征(PMWS)病料中扩增出PCV-2基因组DNA,将基因片段克隆于pMD18-T载体,筛选获得含有相应片段的阳性重组质粒pMD18-T-PCV-2。对筛选出的阳性质粒进行测序。结果表明,PCV山东株的全基因组为1 767 bp,与国内外毒株核苷酸同源性高达99.6%。
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| 关 键 词： | 猪圆环病毒2型 全基因组 克隆 序列分析 |
| 文章编号： | 1007-5038（2008）01-0013-05 |
| 收稿时间： | 2007-09-14 |
| 修稿时间： | 2007-09-14 |
Cloning and Sequence Analysis of Whole Genome of Porcine circovirus type 2(PCV-2) SD Strain |
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| LIU Mei-ling,QIN Xiao-bing,WANG Ai-hua,NIU Jin-guo,SHAN Hu. Cloning and Sequence Analysis of Whole Genome of Porcine circovirus type 2(PCV-2) SD Strain[J]. Progress In Veterinary Medicine, 2008, 29(1): 13-17 |
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| Authors: | LIU Mei-ling QIN Xiao-bing WANG Ai-hua NIU Jin-guo SHAN Hu |
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| Abstract: | A pair of specific primers which were designed according to the published genome sequences of PCV-2 were synthesized to amplify the full length of viral genome from clinically suspected PMWS samples.The gene fragment was cloned into pMD-18-T vector forming the recombinant plasmid pMD-18T-PCV-2.The sequence analysis showed that SD strain genome consisted of 1 767 bp,which shares 99.6% sequence identity with other PCV-2 strains in GenBank. |
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| Keywords: | Porcine circovirus type 2(PCV-2) genome cloning sequence analysis |
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