4种固化剂对若干草坪草愈伤组织诱导的影响

 比较了Phytagel、Canageenen (卡拉胶) 、Agar A 和Agar powder对匍匐翦股颖(Agrostis palustris Huds. ) 品种‘L293’; 草地早熟禾(Poa pratensis L. ) 品种‘佛特纳’; 高羊茅( Festuca arundinacea Schreb) 品种‘新秀’、‘翠碧’; 结缕草( Zoysia japonica Steud. ) 品种‘青岛结缕草’、‘Zenith’和‘松南结缕草’及普通狗牙根(Cynodon dactylon L. ) 品种‘佳宝’成熟种子愈伤组织诱导和胚性愈伤组织发生的影响。结果表明, 就匍匐翦股颖、草地早熟禾而言, 无论是愈伤组织诱导率还是胚性愈伤组织发生率, 均以在Phytagel固化的培养基中最高。高羊茅在Agar powder上愈伤组织诱导率最高, 但就胚性愈伤发生而言固化剂之间无显著差异。虽然‘Zenith’和‘松南结缕草’在愈伤组织诱导上4种固化剂无显著差异, 但就胚性愈伤组织发生而言, ‘青岛结缕草’、‘Zenith’和‘松南结缕草’依次在Phytagel、Canageenen和Agar powder为佳。只有在Phytagel固化的培养基上普通狗牙根形成了胚性愈伤组织。

Effect of Four Brands of Gelling Agents on Callus Induction from Mature Seeds of Several Turfgrass Species

The effects of Phytagel, Canageenen, Agar A and Agar powder on callus induction and embryogenic callus development from the mature seeds of Agrostis palustris‘L293’, Poa pratensis ‘Fortuna’,Festuca arundinacea‘Starlet’and ‘Triple’, Zoysia japonica‘Qingdao’, ‘Zenith’and ‘Songnan’and Cynodon dactylon ‘Jackpot’were systematically compared. The results indicated that both the percentages of callus induction and the percentages of embryogenic callus development were the highest for Agrostis palustris and Poa pratensis on the medium solidified with Phytagel. Although the highest percentage of callus induction was obtained on the Agar powder solidified medium for Festuca arundinacea, no significant differences amongthe four gelling agents were found on the development of emryogenic callus. Although the differences were not significant among the four gelling agents on callus induction from Zoysia japonica ‘Zenith’and ‘Songnan’,the development of embryogenic callus was highest on Phytagel, Canageenen and Agar power for‘Qingdao’,‘Zenith’and ‘Songnan’, respectively. For Cynodon dactylon, embryogenic callus was formed only on the medium solidified with Phytagel.